Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1324347..1324972 Replicon chromosome
Accession NZ_CP085056
Organism Escherichia coli strain P24_WM1_05.20

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag LHK10_RS06430 Protein ID WP_000911330.1
Coordinates 1324574..1324972 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag LHK10_RS06425 Protein ID WP_000450524.1
Coordinates 1324347..1324574 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHK10_RS06400 (1320150) 1320150..1320620 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
LHK10_RS06405 (1320620) 1320620..1321192 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
LHK10_RS06410 (1321338) 1321338..1322216 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
LHK10_RS06415 (1322233) 1322233..1323267 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
LHK10_RS06420 (1323480) 1323480..1324193 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
LHK10_RS06425 (1324347) 1324347..1324574 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
LHK10_RS06430 (1324574) 1324574..1324972 + 399 WP_000911330.1 type II toxin-antitoxin system VapC family toxin Toxin
LHK10_RS06435 (1325119) 1325119..1325982 + 864 WP_001267507.1 neutral zinc metallopeptidase -
LHK10_RS06440 (1325997) 1325997..1328012 + 2016 WP_000829323.1 tRNA cytosine(34) acetyltransferase TmcA -
LHK10_RS06445 (1328086) 1328086..1328784 + 699 WP_000679823.1 esterase -
LHK10_RS06450 (1328894) 1328894..1329094 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14894.25 Da        Isoelectric Point: 9.2216

>T220232 WP_000911330.1 NZ_CP085056:1324574-1324972 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T220232 NZ_CP116987:1297889-1298287 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT220232 WP_000450524.1 NZ_CP085056:1324347-1324574 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT220232 NZ_CP116987:1297662-1297889 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References