Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 321599..322399 Replicon chromosome
Accession NZ_CP085056
Organism Escherichia coli strain P24_WM1_05.20

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag LHK10_RS01505 Protein ID WP_000342449.1
Coordinates 321872..322399 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag LHK10_RS01500 Protein ID WP_001277108.1
Coordinates 321599..321865 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHK10_RS01480 (317256) 317256..317924 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
LHK10_RS01485 (317917) 317917..318975 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
LHK10_RS01490 (319220) 319220..320074 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
LHK10_RS01495 (320346) 320346..321449 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
LHK10_RS01500 (321599) 321599..321865 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
LHK10_RS01505 (321872) 321872..322399 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
LHK10_RS01510 (322396) 322396..322779 - 384 WP_087526099.1 aspartate 1-decarboxylase autocleavage activator PanM -
LHK10_RS01515 (323203) 323203..324312 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
LHK10_RS01520 (324360) 324360..325286 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LHK10_RS01525 (325283) 325283..326560 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
LHK10_RS01530 (326557) 326557..327324 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T220226 WP_000342449.1 NZ_CP085056:321872-322399 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T220226 NZ_CP116987:208748-208855 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT220226 WP_001277108.1 NZ_CP085056:321599-321865 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT220226 NZ_CP116987:c208691-208636 [Escherichia coli]
TCAAGATTAGCCCCCGTTATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References