Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 300308..300894 Replicon chromosome
Accession NZ_CP085056
Organism Escherichia coli strain P24_WM1_05.20

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag LHK10_RS01380 Protein ID WP_042014162.1
Coordinates 300308..300538 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LHK10_RS01385 Protein ID WP_000593555.1
Coordinates 300535..300894 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHK10_RS01370 (296345) 296345..299080 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
LHK10_RS01375 (299080) 299080..300204 + 1125 WP_001314210.1 ABC transporter permease -
LHK10_RS01380 (300308) 300308..300538 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
LHK10_RS01385 (300535) 300535..300894 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LHK10_RS01390 (301014) 301014..301415 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
LHK10_RS01395 (301421) 301421..302227 - 807 WP_226926672.1 nickel import ATP-binding protein NikE -
LHK10_RS01400 (302224) 302224..302988 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
LHK10_RS01405 (302988) 302988..303821 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
LHK10_RS01410 (303818) 303818..304762 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T220225 WP_042014162.1 NZ_CP085056:300308-300538 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T220225 NZ_CP116987:208265-208372 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT220225 WP_000593555.1 NZ_CP085056:300535-300894 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT220225 NZ_CP116987:c208208-208153 [Escherichia coli]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References