Detailed information of TA system TA220223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 513715..514351
• Replicon: chromosome
• Accession: NZ_CP085037
• Organism: Bacillus pumilus strain BIM B-171

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K2MHT4
• Locus tag: LG951_RS02470
• Protein ID: WP_003214169.1
• Coordinates: 514001..514351 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W8QJ31
• Locus tag: LG951_RS02465
• Protein ID: WP_003214273.1
• Coordinates: 513715..513996 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG951_RS02445 (LG951_02445)	509862..510467	-	606	WP_050945548.1	rhomboid family intramembrane serine protease	-
LG951_RS02450 (LG951_02450)	510562..510927	+	366	WP_226804681.1	holo-ACP synthase	-
LG951_RS02455 (LG951_02455)	511088..512104	+	1017	WP_106030172.1	outer membrane lipoprotein carrier protein LolA	-
LG951_RS02460 (LG951_02460)	512240..513421	+	1182	WP_226804682.1	alanine racemase	-
LG951_RS02465 (LG951_02465)	513715..513996	+	282	WP_003214273.1	hypothetical protein	Antitoxin
LG951_RS02470 (LG951_02470)	514001..514351	+	351	WP_003214169.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LG951_RS02475 (LG951_02475)	514466..515296	+	831	WP_226804683.1	RsbT co-antagonist protein RsbRA	-
LG951_RS02480 (LG951_02480)	515301..515669	+	369	WP_003214235.1	RsbT antagonist protein RsbS	-
LG951_RS02485 (LG951_02485)	515672..516073	+	402	WP_003214085.1	anti-sigma regulatory factor	-
LG951_RS02490 (LG951_02490)	516084..517091	+	1008	WP_003213895.1	PP2C family protein-serine/threonine phosphatase	-
LG951_RS02495 (LG951_02495)	517151..517480	+	330	WP_003213847.1	anti-sigma factor antagonist	-
LG951_RS02500 (LG951_02500)	517477..517965	+	489	WP_003214356.1	anti-sigma B factor RsbW	-
LG951_RS02505 (LG951_02505)	517931..518719	+	789	WP_012009000.1	RNA polymerase sigma factor SigB	-
LG951_RS02510 (LG951_02510)	518719..519318	+	600	WP_003214034.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12976.99 Da
• Isoelectric Point: 5.1663

>T220223 WP_003214169.1 NZ_CP085037:514001-514351 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Nucleotide

• Length: 351 bp

>T220223 NZ_CP116984:c23923-23642 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10864.25 Da
• Isoelectric Point: 5.2726

>AT220223 WP_003214273.1 NZ_CP085037:513715-513996 [Bacillus pumilus]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT220223 NZ_CP116984:c24164-23913 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: K2MHT4

Antitoxin

• AlphaFold DB: A0A081L854