Detailed information of TA system TA220219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3337737..3339478
• Replicon: chromosome
• Accession: NZ_CP085032
• Organism: Proteus terrae subsp. cibarius strain ZJ19PC

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LHA39_RS15380
• Protein ID: WP_098941872.1
• Coordinates: 3337737..3339110 (+)
• Length: 458 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LHA39_RS15385
• Protein ID: WP_159241810.1
• Coordinates: 3339107..3339478 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHA39_RS15365 (LHA39_15365)	3333409..3334392	-	984	WP_075673449.1	ABC transporter permease	-
LHA39_RS15370 (LHA39_15370)	3334377..3335960	-	1584	WP_099659717.1	ABC transporter substrate-binding protein	-
LHA39_RS15375 (LHA39_15375)	3336366..3337355	-	990	WP_159241809.1	glycosyltransferase	-
LHA39_RS15380 (LHA39_15380)	3337737..3339110	+	1374	WP_098941872.1	HipA domain-containing protein	Toxin
LHA39_RS15385 (LHA39_15385)	3339107..3339478	+	372	WP_159241810.1	helix-turn-helix domain-containing protein	Antitoxin
LHA39_RS15390 (LHA39_15390)	3339607..3340107	-	501	WP_075673444.1	hydrogenase maturation peptidase HycI	-
LHA39_RS15395 (LHA39_15395)	3340104..3340514	-	411	WP_088494545.1	formate hydrogenlyase maturation HycH family protein	-
LHA39_RS15400 (LHA39_15400)	3340507..3341289	-	783	WP_072069032.1	NADH-quinone oxidoreductase subunit B family protein	-
LHA39_RS15405 (LHA39_15405)	3341286..3341840	-	555	WP_075673442.1	hydrogenase 4 subunit H	-
LHA39_RS15410 (LHA39_15410)	3341852..3343585	-	1734	WP_075673441.1	hydrogenase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (174-418)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-103)

Sequences

Toxin

Protein

• Length: 458 a.a.
• Molecular weight: 52589.44 Da
• Isoelectric Point: 4.6415

>T220219 WP_098941872.1 NZ_CP085032:3337737-3339110 [Proteus terrae subsp. cibarius]
MEKITLQAFLNQEWVDIAIINFPKYDRMLYASKYDITYPITELEYETSYSIEHLYQDDQHAVSINHPVELYFDDSGSPGW
LKFLDDIVPSGASRRYWLQYLDIAELSTEQQNYELLHHGTISPIGNLRVKESLPAPSPLNRNLFFTINDVKNRASDFLDY
AQRKGAAAGGATGAGGEAPKLLLRCNDNEEVWIDTYQDDISNLDKYYLVKFPRGSRSVVDCNILRTEYHFYHELTDMGFD
TIPIKDMRLEEGDNYPSLWLPRFDIKIENNKIIRLAMESVYSILNKAPGTTLYHETVIKELIDKINQSNMVTEFGFQFNT
ANFIIEWVRRDLLNIIFGNSDNHGRNTSLIRYNNAIYLAPIYDFAPMKADPEGIARTTKWGSHSEIGGEYNFINIADSLS
DLIAPEQLLNALQETAQQLLTLKERLEMRGVPTQILTMPSFGFDFIPNKLERWGLLP

Nucleotide

• Length: 1374 bp

>T220219 NZ_CP116981:4725726-4726037 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13856.12 Da
• Isoelectric Point: 10.6462

>AT220219 WP_159241810.1 NZ_CP085032:3339107-3339478 [Proteus terrae subsp. cibarius]
MNNVENKNHIDKFPTRASRKHQFVATTDRKQVISKIMAHLLFGEITQGEALKMLRIKVLGLNQDLYAKLINVSRKTLSEI
ENDRGNYSADIINKVFKPFGLKVGLVPSSPHILSSLLKESPSN

Nucleotide

• Length: 372 bp

>AT220219 NZ_CP116981:4726038-4726328 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG