Detailed information of TA system TA220218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/GNAT-DUF1778
• Location: 2355713..2356458
• Replicon: chromosome
• Accession: NZ_CP085032
• Organism: Proteus terrae subsp. cibarius strain ZJ19PC

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LHA39_RS10965
• Protein ID: WP_159241970.1
• Coordinates: 2355967..2356458 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LHA39_RS10960
• Protein ID: WP_075673024.1
• Coordinates: 2355713..2355979 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHA39_RS10925 (LHA39_10925)	2351374..2351856	+	483	WP_006533151.1	flagellar basal body-associated protein FliL	-
LHA39_RS10930 (LHA39_10930)	2351862..2352893	+	1032	WP_023581831.1	flagellar motor switch protein FliM	-
LHA39_RS10935 (LHA39_10935)	2352886..2353296	+	411	WP_006533153.1	flagellar motor switch protein FliN	-
LHA39_RS10940 (LHA39_10940)	2353300..2353746	+	447	WP_075673022.1	flagellar biosynthetic protein FliO	-
LHA39_RS10945 (LHA39_10945)	2353746..2354516	+	771	WP_023581828.1	flagellar type III secretion system pore protein FliP	-
LHA39_RS10950 (LHA39_10950)	2354532..2354801	+	270	WP_069367481.1	flagellar biosynthesis protein FliQ	-
LHA39_RS10955 (LHA39_10955)	2354807..2355589	+	783	WP_099660619.1	flagellar biosynthetic protein FliR	-
LHA39_RS10960 (LHA39_10960)	2355713..2355979	+	267	WP_075673024.1	DUF1778 domain-containing protein	Antitoxin
LHA39_RS10965 (LHA39_10965)	2355967..2356458	+	492	WP_159241970.1	GNAT family N-acetyltransferase	Toxin
LHA39_RS10970 (LHA39_10970)	2356544..2357488	-	945	WP_159241969.1	flagellar hook-associated protein FlgL	-
LHA39_RS10975 (LHA39_10975)	2357520..2359163	-	1644	WP_159241968.1	flagellar hook-associated protein FlgK	-
LHA39_RS10980 (LHA39_10980)	2359281..2360267	-	987	WP_159241967.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
LHA39_RS10985 (LHA39_10985)	2360267..2361373	-	1107	WP_159241966.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18240.21 Da
• Isoelectric Point: 10.3194

>T220218 WP_159241970.1 NZ_CP085032:2355967-2356458 [Proteus terrae subsp. cibarius]
MGKVTAPEPLSNSHEVADFYSSENVLDNWIKQRGLKNQFLGASRTFVVCEKNKKRVVGYYSLATGSVNHTEAMSHIRRNM
PDPIPVIILARLAVDKTFHGKGLGADLLRDAVLRCHHVAENIGVRAIMVHALTENAKHFYLHHGFKASSIQDKILFLALK
KKI

Nucleotide

• Length: 492 bp

>T220218 NZ_CP116981:c4230901-4230626 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10111.62 Da
• Isoelectric Point: 4.5256

>AT220218 WP_075673024.1 NZ_CP085032:2355713-2355979 [Proteus terrae subsp. cibarius]
MKADVQLNIRAKESQRALIDTAAEILHKSRTDFILETACQAAEDVILDRRTFNLNDAQYAEFIEIIDAPVDIDPALEKLL
MRKPLWEK

Nucleotide

• Length: 267 bp

>AT220218 NZ_CP116981:c4230629-4230270 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA