Detailed information of TA system TA220217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 60845..61370
• Replicon: plasmid pKP18-2110-2-2
• Accession: NZ_CP084988
• Organism: Klebsiella pneumoniae strain KP18-2110-2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: LH404_RS28320
• Protein ID: WP_013023785.1
• Coordinates: 61065..61370 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: LH404_RS28315
• Protein ID: WP_001568025.1
• Coordinates: 60845..61063 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH404_RS28285	56335..57024	+	690	WP_020315560.1	RES family NAD+ phosphorylase	-
LH404_RS28290	57056..57745	-	690	WP_004193995.1	hypothetical protein	-
LH404_RS28295	58012..58893	+	882	WP_223289722.1	hypothetical protein	-
LH404_RS28300	58995..59243	+	249	WP_053389907.1	hypothetical protein	-
LH404_RS28305	59297..60283	-	987	WP_223289723.1	hypothetical protein	-
LH404_RS28310	60421..60675	+	255	Protein_59	hypothetical protein	-
LH404_RS28315	60845..61063	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LH404_RS28320	61065..61370	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
LH404_RS28325	61539..61934	+	396	WP_162269742.1	hypothetical protein	-
LH404_RS28330	61961..62275	+	315	WP_053389906.1	hypothetical protein	-
LH404_RS28335	62286..63302	+	1017	WP_017899884.1	hypothetical protein	-
LH404_RS28340	63500..64294	+	795	WP_053389905.1	site-specific integrase	-
LH404_RS28345	64726..65028	-	303	WP_004197636.1	hypothetical protein	-
LH404_RS28350	65025..65651	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..142244	142244

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T220217 WP_013023785.1 NZ_CP084988:61065-61370 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T220217 NZ_CP116981:3911527-3911991 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT220217 WP_001568025.1 NZ_CP084988:60845-61063 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT220217 NZ_CP116981:3911192-3911527 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8