Detailed information of TA system TA220216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 337202..337959
• Replicon: plasmid pKP18-2110-2-1
• Accession: NZ_CP084987
• Organism: Klebsiella pneumoniae strain KP18-2110-2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J4ZXV7
• Locus tag: LH404_RS27915
• Protein ID: WP_029497408.1
• Coordinates: 337202..337684 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LH404_RS27920
• Protein ID: WP_094645258.1
• Coordinates: 337672..337959 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH404_RS27885	332234..332449	-	216	WP_009653220.1	hypothetical protein	-
LH404_RS27890	333984..334361	+	378	WP_042927811.1	hypothetical protein	-
LH404_RS27895	334724..335695	-	972	WP_009653249.1	hypothetical protein	-
LH404_RS27900	336234..336494	+	261	WP_009653224.1	hypothetical protein	-
LH404_RS27905	336702..336821	-	120	WP_223884740.1	Hok/Gef family protein	-
LH404_RS27910	336769..336921	-	153	Protein_351	DUF5431 family protein	-
LH404_RS27915	337202..337684	-	483	WP_029497408.1	GNAT family N-acetyltransferase	Toxin
LH404_RS27920	337672..337959	-	288	WP_094645258.1	DUF1778 domain-containing protein	Antitoxin
LH404_RS27925	338196..339119	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
LH404_RS27930	339319..339891	-	573	WP_094645259.1	cytochrome b/b6 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ARR-3 / dfrA27 / aadA16 / qacE / sul1 / blaNDM-1 / mph(A) / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	katA	1..362034	362034
	-	flank	IS/Tn	-	-	340417..341220	803

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17599.34 Da
• Isoelectric Point: 9.8719

>T220216 WP_029497408.1 NZ_CP084987:c337684-337202 [Klebsiella pneumoniae]
VGRLTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLRLP

Nucleotide

• Length: 483 bp

>T220216 NZ_CP116981:c3660084-3659677 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10955.66 Da
• Isoelectric Point: 4.5141

>AT220216 WP_094645258.1 NZ_CP084987:c337959-337672 [Klebsiella pneumoniae]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVVDD
PVIEKLLARKPQWDV

Nucleotide

• Length: 288 bp

>AT220216 NZ_CP116981:c3660331-3660065 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZXV7