Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5252469..5253094 Replicon chromosome
Accession NZ_CP084986
Organism Klebsiella pneumoniae strain KP18-2110-2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LH404_RS25790 Protein ID WP_002882817.1
Coordinates 5252469..5252852 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LH404_RS25795 Protein ID WP_004150355.1
Coordinates 5252852..5253094 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH404_RS25775 5249835..5250737 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LH404_RS25780 5250734..5251369 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LH404_RS25785 5251366..5252295 + 930 WP_193525095.1 formate dehydrogenase accessory protein FdhE -
LH404_RS25790 5252469..5252852 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LH404_RS25795 5252852..5253094 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LH404_RS25800 5253299..5254216 + 918 WP_002882812.1 alpha/beta hydrolase -
LH404_RS25805 5254230..5255171 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LH404_RS25810 5255216..5255653 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LH404_RS25815 5255650..5256510 - 861 WP_002882807.1 virulence factor BrkB family protein -
LH404_RS25820 5256504..5257103 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T220214 WP_002882817.1 NZ_CP084986:c5252852-5252469 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T220214 NZ_CP116981:c3229104-3228706 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT220214 WP_004150355.1 NZ_CP084986:c5253094-5252852 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT220214 NZ_CP116981:c3229331-3229104 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References