Detailed information of TA system TA220209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2709382..2710970
• Replicon: chromosome
• Accession: NZ_CP084986
• Organism: Klebsiella pneumoniae strain KP18-2110-2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0H3GN97
• Locus tag: LH404_RS13425
• Protein ID: WP_004199851.1
• Coordinates: 2709382..2710704 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: LH404_RS13430
• Protein ID: WP_002904790.1
• Coordinates: 2710704..2710970 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH404_RS13410	2704447..2706948	+	2502	WP_004199853.1	fimbria/pilus outer membrane usher protein	-
LH404_RS13415	2706936..2707940	+	1005	WP_004199852.1	fimbrial protein	-
LH404_RS13420	2708073..2709365	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LH404_RS13425	2709382..2710704	-	1323	WP_004199851.1	type II toxin-antitoxin system HipA family toxin	Toxin
LH404_RS13430	2710704..2710970	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LH404_RS13435	2711236..2711562	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
LH404_RS13440	2711559..2712059	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LH404_RS13445	2712037..2713416	-	1380	Protein_2633	nucleobase:cation symporter-2 family protein	-
LH404_RS13450	2713762..2714916	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LH404_RS13455	2714897..2715820	-	924	WP_004199849.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49128.44 Da
• Isoelectric Point: 7.2487

>T220209 WP_004199851.1 NZ_CP084986:c2710704-2709382 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPLEQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T220209 NZ_CP116981:2117656-2117832 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT220209 WP_002904790.1 NZ_CP084986:c2710970-2710704 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT220209 NZ_CP116981:2117878-2118294 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GN97

Antitoxin

• AlphaFold DB: A0A0H3GXD8