Detailed information of TA system TA220195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 195458..196128
• Replicon: plasmid pPAPIvir
• Accession: NZ_CP084985
• Organism: Klebsiella pneumoniae strain KP-PA

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: KYT91_RS27365
• Protein ID: WP_004213072.1
• Coordinates: 195458..195901 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: KYT91_RS27370
• Protein ID: WP_004213073.1
• Coordinates: 195898..196128 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYT91_RS27330 (KYT91_0970)	190865..191140	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
KYT91_RS27335 (KYT91_0975)	191203..191694	+	492	WP_011251327.1	DM13 domain-containing protein	-
KYT91_RS27340	191743..192663	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
KYT91_RS27345	192754..193157	+	404	Protein_212	GAF domain-containing protein	-
KYT91_RS27350	193676..194314	-	639	Protein_213	mucoid phenotype regulator RmpA2	-
KYT91_RS27355	194731..195035	+	305	Protein_214	transposase	-
KYT91_RS27360	195058..195309	-	252	WP_186987481.1	hypothetical protein	-
KYT91_RS27365 (KYT91_0990)	195458..195901	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
KYT91_RS27370 (KYT91_0995)	195898..196128	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KYT91_RS27375 (KYT91_1010)	196736..197869	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
KYT91_RS27380 (KYT91_1015)	197885..198178	+	294	WP_004213076.1	hypothetical protein	-
KYT91_RS27385	198168..198374	-	207	WP_004213077.1	hypothetical protein	-
KYT91_RS27390 (KYT91_1020)	198726..199016	+	291	WP_004213078.1	hypothetical protein	-
KYT91_RS27395 (KYT91_1025)	199006..199905	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(3')-Ia / mph(A) / sul1 / qacE / dfrA5 / mph(E) / msr(E) / armA / sul2 / blaCTX-M-15	rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..382377	382377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T220195 WP_004213072.1 NZ_CP084985:c195901-195458 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T220195 NZ_CP116977:2265-2540 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT220195 WP_004213073.1 NZ_CP084985:c196128-195898 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT220195 NZ_CP116977:1981-2265 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620