Detailed information of TA system TA220194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 77494..78404
• Replicon: plasmid pPAPIvir
• Accession: NZ_CP084985
• Organism: Klebsiella pneumoniae strain KP-PA

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: KYT91_RS26740
• Protein ID: WP_004026354.1
• Coordinates: 77934..78404 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: KYT91_RS26735
• Protein ID: WP_004181895.1
• Coordinates: 77494..77937 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYT91_RS26720 (KYT91_0345)	74127..75614	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
KYT91_RS26725 (KYT91_0350)	75700..76122	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
KYT91_RS26730 (KYT91_0355)	76122..77393	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
KYT91_RS26735 (KYT91_0360)	77494..77937	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
KYT91_RS26740 (KYT91_0365)	77934..78404	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
KYT91_RS26745 (KYT91_0370)	78514..78774	-	261	WP_004026352.1	hypothetical protein	-
KYT91_RS26750	79496..79654	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
KYT91_RS26755	80257..81231	+	975	WP_058842863.1	hypothetical protein	-
KYT91_RS28405	81215..81337	+	123	WP_257183836.1	hypothetical protein	-
KYT91_RS26760 (KYT91_0375)	81429..81806	-	378	WP_029884711.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(3')-Ia / mph(A) / sul1 / qacE / dfrA5 / mph(E) / msr(E) / armA / sul2 / blaCTX-M-15	rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..382377	382377
	-	flank	IS/Tn	-	-	72462..73682	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T220194 WP_004026354.1 NZ_CP084985:77934-78404 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T220194 NZ_CP116976:c4570083-4569697 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT220194 WP_004181895.1 NZ_CP084985:77494-77937 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT220194 NZ_CP116976:c4569707-4569378 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A2J4RLY9