Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5290442..5291067 Replicon chromosome
Accession NZ_CP084984
Organism Klebsiella pneumoniae strain KP-PA

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KYT91_RS25870 Protein ID WP_002882817.1
Coordinates 5290442..5290825 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KYT91_RS25875 Protein ID WP_004150355.1
Coordinates 5290825..5291067 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYT91_RS25855 (5287808) 5287808..5288710 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KYT91_RS25860 (5288707) 5288707..5289342 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KYT91_RS25865 (5289339) 5289339..5290268 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KYT91_RS25870 (5290442) 5290442..5290825 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KYT91_RS25875 (5290825) 5290825..5291067 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KYT91_RS25880 (5291272) 5291272..5292189 + 918 WP_021466676.1 alpha/beta hydrolase -
KYT91_RS25885 (5292203) 5292203..5293144 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KYT91_RS25890 (5293189) 5293189..5293626 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KYT91_RS25895 (5293623) 5293623..5294483 - 861 WP_004146232.1 virulence factor BrkB family protein -
KYT91_RS25900 (5294477) 5294477..5295076 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T220193 WP_002882817.1 NZ_CP084984:c5290825-5290442 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T220193 NZ_CP116976:c4183220-4183002 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT220193 WP_004150355.1 NZ_CP084984:c5291067-5290825 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT220193 NZ_CP116976:c4183620-4183246 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References