Detailed information of TA system TA022019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 3559134..3559762
• Replicon: chromosome
• Accession: NC_009380
• Organism: Salinispora tropica CNB-440

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A4X9G8
• Locus tag: STROP_RS15555
• Protein ID: WP_012014319.1
• Coordinates: 3559134..3559517 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: STROP_RS15560
• Protein ID: WP_018829262.1
• Coordinates: 3559514..3559762 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
STROP_RS15530	3555144..3555347	-	204	WP_018833060.1	DUF397 domain-containing protein	-
STROP_RS15535	3555371..3556225	-	855	WP_012014316.1	helix-turn-helix transcriptional regulator	-
STROP_RS15540	3556377..3556910	+	534	WP_012014317.1	hypothetical protein	-
STROP_RS15545	3556907..3557203	+	297	WP_187151642.1	hypothetical protein	-
STROP_RS15550	3557214..3559055	+	1842	WP_012014318.1	DNA helicase RecQ	-
STROP_RS15555	3559134..3559517	-	384	WP_012014319.1	PIN domain-containing protein	Toxin
STROP_RS15560	3559514..3559762	-	249	WP_018829262.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
STROP_RS15565	3559830..3560672	-	843	WP_012014321.1	alpha/beta hydrolase	-
STROP_RS15570	3560712..3562709	-	1998	WP_012014322.1	excinuclease ABC subunit UvrC	-
STROP_RS15575	3562780..3563280	-	501	WP_012014323.1	Rieske (2Fe-2S) protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (21-115)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-61)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13558.60 Da
• Isoelectric Point: 5.7101

>T22019 WP_012014319.1 NC_009380:c3559517-3559134 [Salinispora tropica CNB-440]
VSRGILDTSILIATDVTPIPGELAISVTSLAELQFGVLAAKTADARAGRLARLSAIQRRFDPLPIDEAVADSYARLAARV
VEIGRQPRARVMDLLIAATAHAHDAAVYTRNADDFAGLEDLVLIRTI

Nucleotide

• Length: 384 bp

>T22019 NC_009380:c3559517-3559134 [Salinispora tropica CNB-440]
GTGAGTCGCGGCATCCTCGACACCAGCATCCTCATTGCTACTGATGTAACCCCGATTCCCGGCGAACTGGCCATCAGCGT
CACTAGCCTTGCCGAGCTCCAGTTCGGGGTGCTGGCAGCGAAAACCGCTGACGCGAGGGCGGGTCGTCTAGCGCGGTTGA
GTGCCATCCAACGTCGATTCGATCCCCTTCCCATCGACGAGGCCGTTGCTGACAGCTATGCGCGATTAGCCGCACGTGTG
GTCGAGATTGGCCGCCAACCGCGAGCCAGGGTGATGGACCTGCTCATCGCCGCGACCGCACACGCTCACGATGCTGCCGT
CTACACCCGCAACGCCGATGATTTCGCTGGTCTCGAGGACCTCGTCCTGATCCGTACGATCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9049.09 Da
• Isoelectric Point: 4.7223

>AT22019 WP_018829262.1 NC_009380:c3559762-3559514 [Salinispora tropica CNB-440]
MDEVGLREMRQNASDLVRRAQAGERVTITVAGRPAAVLGPVGPRAWRGWDDIASVFQQPTDPGWAADRHLLDDAVTDPWA
ER

Nucleotide

• Length: 249 bp

>AT22019 NC_009380:c3559762-3559514 [Salinispora tropica CNB-440]
ATGGACGAGGTCGGGCTACGGGAGATGCGGCAGAACGCCAGCGACTTGGTACGTCGGGCACAAGCGGGTGAACGCGTGAC
GATTACCGTCGCCGGTCGGCCAGCAGCCGTGCTCGGCCCGGTCGGTCCGCGTGCCTGGCGCGGGTGGGACGACATTGCCA
GCGTGTTCCAGCAGCCCACGGACCCCGGATGGGCAGCGGATCGGCACCTTCTCGACGACGCTGTCACCGACCCGTGGGCT
GAGCGGTGA

Structures

Toxin

• AlphaFold DB: A4X9G8