Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1468591..1469327 Replicon chromosome
Accession NZ_CP084984
Organism Klebsiella pneumoniae strain KP-PA

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag KYT91_RS07330 Protein ID WP_032433360.1
Coordinates 1468845..1469327 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KYT91_RS07325 Protein ID WP_003026799.1
Coordinates 1468591..1468857 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYT91_RS07300 (1464237) 1464237..1465376 + 1140 WP_032433368.1 mannitol dehydrogenase -
KYT91_RS07305 (1465405) 1465405..1466067 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
KYT91_RS07310 (1466051) 1466051..1467055 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
KYT91_RS07315 (1467073) 1467073..1467705 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
KYT91_RS07320 (1467715) 1467715..1468278 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
KYT91_RS07325 (1468591) 1468591..1468857 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KYT91_RS07330 (1468845) 1468845..1469327 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
KYT91_RS07335 (1469527) 1469527..1470930 + 1404 WP_001272054.1 ISNCY-like element ISKpn21 family transposase -
KYT91_RS07340 (1470959) 1470959..1471591 - 633 WP_001567369.1 hypothetical protein -
KYT91_RS07345 (1471971) 1471971..1472570 - 600 WP_064472304.1 helix-turn-helix transcriptional regulator -
KYT91_RS07350 (1472783) 1472783..1473727 - 945 WP_077254249.1 fimbrial protein -
KYT91_RS07355 (1473739) 1473739..1474317 - 579 WP_032432061.1 type 1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1438751..1483618 44867
- flank IS/Tn - - 1469527..1470930 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T220179 WP_032433360.1 NZ_CP084984:1468845-1469327 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T220179 NZ_CP116976:c1235968-1235714 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT220179 WP_003026799.1 NZ_CP084984:1468591-1468857 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT220179 NZ_CP116976:c1236216-1235965 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References