Detailed information of TA system TA220173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 30727..31288
• Replicon: plasmid pDSM110277_d
• Accession: NZ_CP084963
• Organism: Sulfitobacter pontiacus strain DSM 110277

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DSM110277_RS18050
• Protein ID: WP_243252184.1
• Coordinates: 31013..31288 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1N7QLX2
• Locus tag: DSM110277_RS18045
• Protein ID: WP_044353023.1
• Coordinates: 30727..31026 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSM110277_RS18020 (DSM110277_03622)	26468..26677	+	210	WP_243252181.1	DUF6330 family protein	-
DSM110277_RS18025 (DSM110277_03623)	26766..27155	+	390	WP_243252182.1	DUF3768 domain-containing protein	-
DSM110277_RS18030 (DSM110277_03624)	27312..29294	+	1983	WP_243252183.1	ParB/RepB/Spo0J family partition protein	-
DSM110277_RS18035 (DSM110277_03625)	29373..29966	+	594	WP_132998379.1	regulator	-
DSM110277_RS18040 (DSM110277_03626)	29966..30622	+	657	WP_132998378.1	hypothetical protein	-
DSM110277_RS18045 (DSM110277_03627)	30727..31026	+	300	WP_044353023.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DSM110277_RS18050 (DSM110277_03628)	31013..31288	+	276	WP_243252184.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DSM110277_RS18055 (DSM110277_03629)	31395..35657	+	4263	WP_243252185.1	strawberry notch family protein	-
DSM110277_RS18060 (DSM110277_03630)	35779..36165	+	387	WP_132998375.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..128813	128813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-54)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10447.99 Da
• Isoelectric Point: 4.7914

>T220173 WP_243252184.1 NZ_CP084963:31013-31288 [Sulfitobacter pontiacus]
MRNLEWKATAIADLLAIIDYISDDNPDAAQVLKDEIEHKTSLLPERPQMYRAGRVDGTREMVVRPNYIVVYAESPEMVTI
LRVLHAAQQWP

Nucleotide

• Length: 276 bp

>T220173 NZ_CP116975:c53331-53050 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10462.91 Da
• Isoelectric Point: 5.6448

>AT220173 WP_044353023.1 NZ_CP084963:30727-31026 [Sulfitobacter pontiacus]
MPAQTSMLHVRVDDQLKAQASDALAGVGLTLSDAVRILLTRVAAEGGLPAGLTADPDAYDAWFRAKVQEALDDPRPTTPH
AKAMQDAQALIDGKRLAKS

Nucleotide

• Length: 300 bp

>AT220173 NZ_CP116975:c53572-53321 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1N7QLX2