Detailed information of TA system TA220171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 83510..84035
• Replicon: plasmid pCEP14_03
• Accession: NZ_CP084924
• Organism: Acinetobacter pittii strain CEP14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LG653_RS20265
• Protein ID: WP_065996230.1
• Coordinates: 83510..83797 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LG653_RS20270
• Protein ID: WP_005130375.1
• Coordinates: 83787..84035 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG653_RS20235 (LG653_20235)	78722..79510	+	789	WP_068558565.1	OmpA family protein	-
LG653_RS20240 (LG653_20240)	79756..80586	-	831	Protein_64	IS3 family transposase	-
LG653_RS20245 (LG653_20245)	80870..81604	+	735	WP_071211297.1	hypothetical protein	-
LG653_RS20250 (LG653_20250)	81635..81967	+	333	WP_005253224.1	hypothetical protein	-
LG653_RS20255 (LG653_20255)	82142..82707	-	566	Protein_67	recombinase family protein	-
LG653_RS20260 (LG653_20260)	83200..83349	+	150	WP_079878061.1	DUF4113 domain-containing protein	-
LG653_RS20265 (LG653_20265)	83510..83797	-	288	WP_065996230.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LG653_RS20270 (LG653_20270)	83787..84035	-	249	WP_005130375.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LG653_RS20275 (LG653_20275)	84038..84463	-	426	WP_065996232.1	hypothetical protein	-
LG653_RS20280 (LG653_20280)	84546..84614	-	69	Protein_72	helix-turn-helix domain-containing protein	-
LG653_RS20285 (LG653_20285)	84730..84969	-	240	WP_005130377.1	hypothetical protein	-
LG653_RS20290 (LG653_20290)	84966..85595	-	630	WP_005130378.1	AAA family ATPase	-
LG653_RS20295 (LG653_20295)	86024..86938	+	915	WP_004843977.1	replication initiation protein RepM	-
LG653_RS20300 (LG653_20300)	87528..88571	+	1044	WP_226789445.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89672	89672
	-	flank	IS/Tn	-	-	79756..80610	854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11160.07 Da
• Isoelectric Point: 10.3011

>T220171 WP_065996230.1 NZ_CP084924:c83797-83510 [Acinetobacter pittii]
MTYKLDFLEEALAEWKKLNPSIKQPLKKKLIKVLENPRIPKNKLPGHPNRYKIKLRSVGYRLVYEVIDDEVIFIVIAVGR
RENNAVYDEANSRHS

Nucleotide

• Length: 288 bp

>T220171 NZ_CP116972:55544-55696 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9258.77 Da
• Isoelectric Point: 5.0491

>AT220171 WP_005130375.1 NZ_CP084924:c84035-83787 [Acinetobacter pittii]
MTQLIHARMTASITELKKSPMDTVLAGKGEPVAILNRNIPAFYCVPADLYETMMEQLEDLELNKIADARANQKRIRVNID
DL

Nucleotide

• Length: 249 bp

>AT220171 NZ_CP116972:c55494-55432 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT