Detailed information of TA system TA220169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 38671..39196
• Replicon: plasmid pCEP14_03
• Accession: NZ_CP084924
• Organism: Acinetobacter pittii strain CEP14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LG653_RS20075
• Protein ID: WP_065996230.1
• Coordinates: 38671..38958 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LG653_RS20080
• Protein ID: WP_005130375.1
• Coordinates: 38948..39196 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG653_RS20050 (LG653_20050)	33883..34671	+	789	WP_068558565.1	OmpA family protein	-
LG653_RS20055 (LG653_20055)	34917..35747	-	831	Protein_27	IS3 family transposase	-
LG653_RS20060 (LG653_20060)	36031..36765	+	735	WP_071211297.1	hypothetical protein	-
LG653_RS20065 (LG653_20065)	37302..37868	-	567	WP_004282045.1	recombinase family protein	-
LG653_RS20070 (LG653_20070)	38361..38510	+	150	WP_079878061.1	DUF4113 domain-containing protein	-
LG653_RS20075 (LG653_20075)	38671..38958	-	288	WP_065996230.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LG653_RS20080 (LG653_20080)	38948..39196	-	249	WP_005130375.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LG653_RS20085 (LG653_20085)	39199..39624	-	426	WP_065996232.1	hypothetical protein	-
LG653_RS20090 (LG653_20090)	39707..39775	-	69	Protein_34	helix-turn-helix domain-containing protein	-
LG653_RS20095 (LG653_20095)	39891..40130	-	240	WP_005130377.1	hypothetical protein	-
LG653_RS20100 (LG653_20100)	40127..40756	-	630	WP_005130378.1	AAA family ATPase	-
LG653_RS20105 (LG653_20105)	41185..42099	+	915	WP_004843977.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89672	89672
	-	flank	IS/Tn	-	-	34917..35771	854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11160.07 Da
• Isoelectric Point: 10.3011

>T220169 WP_065996230.1 NZ_CP084924:c38958-38671 [Acinetobacter pittii]
MTYKLDFLEEALAEWKKLNPSIKQPLKKKLIKVLENPRIPKNKLPGHPNRYKIKLRSVGYRLVYEVIDDEVIFIVIAVGR
RENNAVYDEANSRHS

Nucleotide

• Length: 288 bp

>T220169 NZ_CP116971:c152890-152585 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTCATTGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCTATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9258.77 Da
• Isoelectric Point: 5.0491

>AT220169 WP_005130375.1 NZ_CP084924:c39196-38948 [Acinetobacter pittii]
MTQLIHARMTASITELKKSPMDTVLAGKGEPVAILNRNIPAFYCVPADLYETMMEQLEDLELNKIADARANQKRIRVNID
DL

Nucleotide

• Length: 249 bp

>AT220169 NZ_CP116971:c153110-152892 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA