Detailed information of TA system TA220168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 17382..17910
• Replicon: plasmid pCEP14_03
• Accession: NZ_CP084924
• Organism: Acinetobacter pittii strain CEP14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A4R0ERS7
• Locus tag: LG653_RS20000
• Protein ID: WP_038349496.1
• Coordinates: 17382..17675 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9B5Q7
• Locus tag: LG653_RS20005
• Protein ID: WP_000246756.1
• Coordinates: 17665..17910 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG653_RS19970 (LG653_19970)	12799..13503	-	705	Protein_10	IS6-like element IS1006 family transposase	-
LG653_RS19975 (LG653_19975)	13663..14967	-	1305	WP_068558395.1	Y-family DNA polymerase	-
LG653_RS19980 (LG653_19980)	14983..15561	-	579	WP_068520013.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
LG653_RS19985 (LG653_19985)	15733..15891	+	159	Protein_13	IS6 family transposase	-
LG653_RS19990 (LG653_19990)	15932..16234	-	303	WP_001140620.1	XRE family transcriptional regulator	-
LG653_RS19995 (LG653_19995)	16227..16583	-	357	WP_004668307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LG653_RS20000 (LG653_20000)	17382..17675	-	294	WP_038349496.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LG653_RS20005 (LG653_20005)	17665..17910	-	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LG653_RS20010 (LG653_20010)	18116..18754	+	639	WP_071211298.1	DUF4263 domain-containing protein	-
LG653_RS20015 (LG653_20015)	18847..19401	+	555	WP_226789440.1	hypothetical protein	-
LG653_RS20025 (LG653_20025)	20584..20730	-	147	Protein_21	resolvase	-
LG653_RS20030 (LG653_20030)	20911..21438	-	528	WP_000537572.1	plasmid pRiA4b ORF-3 family protein	-
LG653_RS20035 (LG653_20035)	22098..22759	+	662	Protein_23	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89672	89672
	-	inside	IScluster/Tn	-	-	12799..22712	9913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-71)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11320.54 Da
• Isoelectric Point: 10.5074

>T220168 WP_038349496.1 NZ_CP084924:c17675-17382 [Acinetobacter pittii]
MTYKLLRHKDFTAEWERLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T220168 NZ_CP116971:103676-104074 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9235.61 Da
• Isoelectric Point: 4.3142

>AT220168 WP_000246756.1 NZ_CP084924:c17910-17665 [Acinetobacter pittii]
MATVNFRVDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Nucleotide

• Length: 246 bp

>AT220168 NZ_CP116971:103449-103676 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A4R0ERS7

Antitoxin

• AlphaFold DB: N9B5Q7