220165

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 46476..47061 Replicon plasmid pCEP14_02
Accession NZ_CP084923
Organism Acinetobacter pittii strain CEP14

Toxin (Protein)


Gene name brnT Uniprot ID A0A158LPL9
Locus tag LG653_RS19850 Protein ID WP_002124950.1
Coordinates 46774..47061 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A062GCE4
Locus tag LG653_RS19845 Protein ID WP_005065064.1
Coordinates 46476..46787 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LG653_RS19810 (LG653_19810) 41789..42718 + 930 WP_005065049.1 replication initiation protein RepM -
LG653_RS19815 (LG653_19815) 42797..43327 + 531 WP_005065050.1 plasmid replication DNA-binding protein -
LG653_RS19820 (LG653_19820) 43677..43994 + 318 WP_000900860.1 hypothetical protein -
LG653_RS19825 (LG653_19825) 44515..44859 + 345 WP_005065055.1 type II toxin-antitoxin system RelE/ParE family toxin -
LG653_RS19830 (LG653_19830) 44864..45166 + 303 WP_005065057.1 XRE family transcriptional regulator -
LG653_RS19835 (LG653_19835) 45258..45659 + 402 WP_005065059.1 hypothetical protein -
LG653_RS19840 (LG653_19840) 45711..46385 + 675 WP_005065062.1 HEPN domain-containing protein -
LG653_RS19845 (LG653_19845) 46476..46787 - 312 WP_005065064.1 BrnA antitoxin family protein Antitoxin
LG653_RS19850 (LG653_19850) 46774..47061 - 288 WP_002124950.1 BrnT family toxin Toxin
LG653_RS19855 (LG653_19855) 47574..49061 + 1488 WP_226789433.1 SulP family inorganic anion transporter -
LG653_RS19860 (LG653_19860) 49074..49925 + 852 WP_005065068.1 universal stress protein -
LG653_RS19865 (LG653_19865) 50196..51080 - 885 WP_005065070.1 MobA/MobL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..58452 58452


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11173.58 Da        Isoelectric Point: 4.9093

>T220165 WP_002124950.1 NZ_CP084923:c47061-46774 [Acinetobacter pittii]
MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNGEERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIIS
ARQVTKAERNQYEHG

Download         Length: 288 bp

>T220165 NZ_CP116970:c4363166-4362948 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 12070.80 Da        Isoelectric Point: 9.4651

>AT220165 WP_005065064.1 NZ_CP084923:c46787-46476 [Acinetobacter pittii]
MSMVRYTRKELNEKFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEITDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQ
GKGYQTRMNKILREAMLEDLKNH

Download         Length: 312 bp

>AT220165 NZ_CP116970:c4363566-4363192 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A158LPL9

Antitoxin

Source ID Structure
AlphaFold DB A0A062GCE4

References