Detailed information of TA system TA220163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 34515..35067
• Replicon: plasmid pCEP14_02
• Accession: NZ_CP084923
• Organism: Acinetobacter pittii strain CEP14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LG653_RS19780
• Protein ID: WP_226789424.1
• Coordinates: 34774..35067 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9KCM7
• Locus tag: LG653_RS19775
• Protein ID: WP_005140357.1
• Coordinates: 34515..34790 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG653_RS19755 (LG653_19755)	29946..31104	-	1159	Protein_36	MobA/MobL family protein	-
LG653_RS19760 (LG653_19760)	31409..32035	+	627	WP_226789431.1	hypothetical protein	-
LG653_RS19765 (LG653_19765)	32460..33605	+	1146	WP_226789423.1	hypothetical protein	-
LG653_RS19770 (LG653_19770)	34087..34311	+	225	WP_002072934.1	hypothetical protein	-
LG653_RS19775 (LG653_19775)	34515..34790	+	276	WP_005140357.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LG653_RS19780 (LG653_19780)	34774..35067	+	294	WP_226789424.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LG653_RS19785 (LG653_19785)	35334..35768	-	435	WP_226789432.1	hypothetical protein	-
LG653_RS19790 (LG653_19790)	36427..37912	+	1486	Protein_43	SulP family inorganic anion transporter	-
LG653_RS19795 (LG653_19795)	37925..38776	+	852	WP_005065068.1	universal stress protein	-
LG653_RS19800 (LG653_19800)	39045..39929	-	885	WP_005065070.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..58452	58452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-94)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (9-75)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11339.31 Da
• Isoelectric Point: 6.9786

>T220163 WP_226789424.1 NZ_CP084923:34774-35067 [Acinetobacter pittii]
MTLTIRYKKQFKKDFKRVSSNPKLKKGLLILKDLIDEHLVKTGTVPDEYLPHPLIGEWKPHMECHILPDLLLIWDVDEDN
NELILARIGSHSELLDK

Nucleotide

• Length: 294 bp

>T220163 NZ_CP116970:c4143894-4143616 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10290.65 Da
• Isoelectric Point: 4.6702

>AT220163 WP_005140357.1 NZ_CP084923:34515-34790 [Acinetobacter pittii]
MNTLAKKDEYVRARVPNELKVQAERILKEVGLNTTDAIRLLLTQVVNRGGFPLELRTPNRTTIEAMNSVAEPKKFSSAQE
LFTDLEDDTDD

Nucleotide

• Length: 276 bp

>AT220163 NZ_CP116970:c4144157-4143897 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: N9KCM7