Detailed information of TA system TA220162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 16364..16907
• Replicon: plasmid pCEP14_02
• Accession: NZ_CP084923
• Organism: Acinetobacter pittii strain CEP14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9KJ78
• Locus tag: LG653_RS19675
• Protein ID: WP_000176412.1
• Coordinates: 16617..16907 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LG653_RS19670
• Protein ID: WP_226789434.1
• Coordinates: 16364..16633 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG653_RS19645 (LG653_19645)	11788..12944	-	1157	Protein_14	MobA/MobL family protein	-
LG653_RS19650 (LG653_19650)	13251..14009	+	759	WP_226789426.1	hypothetical protein	-
LG653_RS19655 (LG653_19655)	14303..15448	+	1146	WP_226789423.1	hypothetical protein	-
LG653_RS19660 (LG653_19660)	15521..15763	-	243	WP_001015364.1	hypothetical protein	-
LG653_RS19665 (LG653_19665)	15932..16156	+	225	WP_002072934.1	hypothetical protein	-
LG653_RS19670 (LG653_19670)	16364..16633	+	270	WP_226789434.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LG653_RS19675 (LG653_19675)	16617..16907	+	291	WP_000176412.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LG653_RS19680 (LG653_19680)	16955..17101	-	147	WP_000192351.1	hypothetical protein	-
LG653_RS19685 (LG653_19685)	18141..18458	-	318	WP_005065053.1	hypothetical protein	-
LG653_RS19690 (LG653_19690)	18809..19375	-	567	WP_000858429.1	plasmid replication DNA-binding protein	-
LG653_RS19695 (LG653_19695)	19439..20320	-	882	WP_005804946.1	replication initiation protein RepM	-
LG653_RS19700 (LG653_19700)	20868..21305	-	438	WP_226789427.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..58452	58452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-96)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-83)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11187.12 Da
• Isoelectric Point: 6.9783

>T220162 WP_000176412.1 NZ_CP084923:16617-16907 [Acinetobacter pittii]
MTLTIRYKKQFKKDFKRVSSNPKLKKGLLILKDLIDEHLVKTGTVPDEYLPHPLIGEWKPHMECHILPDLLLIWDVDEDN
NELILARIGSHSELFG

Nucleotide

• Length: 291 bp

>T220162 NZ_CP116970:3747969-3748346 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10172.50 Da
• Isoelectric Point: 4.5112

>AT220162 WP_226789434.1 NZ_CP084923:16364-16633 [Acinetobacter pittii]
ILWQKDEYVRARVPNELKVQAERILKEVGLNTTDAIRLLLTQVVNRGGFPLELRTPNRTTIEAMNSVAEPKKFSSAQELF
TDLEDDTDD

Nucleotide

• Length: 270 bp

>AT220162 NZ_CP116970:3747548-3747922 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCAGATGCATACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCACCCGCAATCACCTCATAA

Structures

Toxin

• AlphaFold DB: N9KJ78