Detailed information of TA system TA220161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 7283..7835
• Replicon: plasmid pCEP14_02
• Accession: NZ_CP084923
• Organism: Acinetobacter pittii strain CEP14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LG653_RS19615
• Protein ID: WP_226789424.1
• Coordinates: 7542..7835 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9KCM7
• Locus tag: LG653_RS19610
• Protein ID: WP_005140357.1
• Coordinates: 7283..7558 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG653_RS19590 (LG653_19590)	2711..3870	-	1160	Protein_3	MobA/MobL family protein	-
LG653_RS19595 (LG653_19595)	4224..4949	+	726	WP_226789422.1	hypothetical protein	-
LG653_RS19600 (LG653_19600)	5228..6373	+	1146	WP_226789423.1	hypothetical protein	-
LG653_RS19605 (LG653_19605)	6855..7079	+	225	WP_002072934.1	hypothetical protein	-
LG653_RS19610 (LG653_19610)	7283..7558	+	276	WP_005140357.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LG653_RS19615 (LG653_19615)	7542..7835	+	294	WP_226789424.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LG653_RS19620 (LG653_19620)	7863..8021	-	159	WP_226789425.1	hypothetical protein	-
LG653_RS19625 (LG653_19625)	8018..8539	-	522	WP_000999915.1	hypothetical protein	-
LG653_RS19630 (LG653_19630)	9064..9381	-	318	WP_005065053.1	hypothetical protein	-
LG653_RS19635 (LG653_19635)	9732..10298	-	567	WP_000858429.1	plasmid replication DNA-binding protein	-
LG653_RS19640 (LG653_19640)	10362..11243	-	882	WP_005804946.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..58452	58452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-94)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (9-75)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11339.31 Da
• Isoelectric Point: 6.9786

>T220161 WP_226789424.1 NZ_CP084923:7542-7835 [Acinetobacter pittii]
MTLTIRYKKQFKKDFKRVSSNPKLKKGLLILKDLIDEHLVKTGTVPDEYLPHPLIGEWKPHMECHILPDLLLIWDVDEDN
NELILARIGSHSELLDK

Nucleotide

• Length: 294 bp

>T220161 NZ_CP116970:3574035-3574208 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10290.65 Da
• Isoelectric Point: 4.6702

>AT220161 WP_005140357.1 NZ_CP084923:7283-7558 [Acinetobacter pittii]
MNTLAKKDEYVRARVPNELKVQAERILKEVGLNTTDAIRLLLTQVVNRGGFPLELRTPNRTTIEAMNSVAEPKKFSSAQE
LFTDLEDDTDD

Nucleotide

• Length: 276 bp

>AT220161 NZ_CP116970:3573711-3574007 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: N9KCM7