Detailed information of TA system TA220157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3223826..3224504
• Replicon: chromosome
• Accession: NZ_CP084921
• Organism: Acinetobacter pittii strain CEP14

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LG653_RS15720
• Protein ID: WP_226789344.1
• Coordinates: 3224322..3224504 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LG653_RS15715
• Protein ID: WP_032006590.1
• Coordinates: 3223826..3224230 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG653_RS15705 (LG653_15705)	3218953..3223020	-	4068	WP_226789343.1	tape measure protein	-
LG653_RS15710 (LG653_15710)	3223145..3223744	-	600	WP_031968591.1	hypothetical protein	-
LG653_RS15715 (LG653_15715)	3223826..3224230	-	405	WP_032006590.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LG653_RS15720 (LG653_15720)	3224322..3224504	-	183	WP_226789344.1	type II toxin-antitoxin system HicA family toxin	Toxin
LG653_RS15725 (LG653_15725)	3224836..3225369	-	534	WP_031968588.1	hypothetical protein	-
LG653_RS15730 (LG653_15730)	3225416..3226345	-	930	WP_226789345.1	phage tail tube protein	-
LG653_RS15735 (LG653_15735)	3226454..3226666	-	213	WP_049069243.1	hypothetical protein	-
LG653_RS15740 (LG653_15740)	3226668..3227066	-	399	WP_226789346.1	hypothetical protein	-
LG653_RS15745 (LG653_15745)	3227068..3227436	-	369	WP_226789347.1	HK97 gp10 family phage protein	-
LG653_RS15750 (LG653_15750)	3227408..3227812	-	405	WP_226789348.1	hypothetical protein	-
LG653_RS15755 (LG653_15755)	3227994..3228362	-	369	WP_226789349.1	glutamate 5-kinase	-
LG653_RS15760 (LG653_15760)	3228362..3228739	-	378	WP_226789350.1	hypothetical protein	-
LG653_RS15765 (LG653_15765)	3228743..3229342	-	600	WP_226789351.1	HeH/LEM domain protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3202588..3252583	49995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6635.83 Da
• Isoelectric Point: 10.7364

>T220157 WP_226789344.1 NZ_CP084921:c3224504-3224322 [Acinetobacter pittii]
VKSLDLVKMIEADGWYLVRVKGSHHHFKHPTKGGLVTIPHPKKDLPNGTVKSILKQAGLN

Nucleotide

• Length: 183 bp

>T220157 NZ_CP116970:2469937-2470401 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.65 Da
• Isoelectric Point: 4.4367

>AT220157 WP_032006590.1 NZ_CP084921:c3224230-3223826 [Acinetobacter pittii]
MLYPIAIERGSDTEAFGVTVPDIPGCFSAGDTLEEAIENVKEAISGHLEILAEDGEEIPLASEVGKFLDDTDYKGMIWAV
TEVDVSRYLGKPEKINVTLPSRLIRKIDDNVGKDKRFKTRSAFLAAGAEKLLHA

Nucleotide

• Length: 405 bp

>AT220157 NZ_CP116970:2469602-2469937 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA