Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 146708..147351 Replicon plasmid pEC22-OXA-1
Accession NZ_CP084902
Organism Escherichia coli strain EC20

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag LHL15_RS25590 Protein ID WP_001034044.1
Coordinates 146935..147351 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag LHL15_RS25585 Protein ID WP_001261286.1
Coordinates 146708..146938 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHL15_RS25565 (143339) 143339..144094 - 756 WP_000852146.1 replication initiation protein RepE -
LHL15_RS25570 (144816) 144816..145622 - 807 WP_000016970.1 site-specific integrase -
LHL15_RS25575 (145623) 145623..145928 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
LHL15_RS25580 (145930) 145930..146148 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
LHL15_RS25585 (146708) 146708..146938 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LHL15_RS25590 (146935) 146935..147351 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
LHL15_RS25595 (147426) 147426..148991 + 1566 WP_001128474.1 AAA family ATPase -
LHL15_RS25600 (148976) 148976..149998 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD iutA / iucD / iucC / iucB / iucA 1..169208 169208
- flank IS/Tn - - 150252..150755 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T220145 WP_001034044.1 NZ_CP084902:146935-147351 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T220145 NZ_CP116963:51321-51662 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGTTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT220145 WP_001261286.1 NZ_CP084902:146708-146938 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT220145 NZ_CP116963:51091-51321 [Enterococcus faecalis]
ATGTTAACAACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References