Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 145623..146148 | Replicon | plasmid pEC22-OXA-1 |
| Accession | NZ_CP084902 | ||
| Organism | Escherichia coli strain EC20 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | S1PFV8 |
| Locus tag | LHL15_RS25575 | Protein ID | WP_001159871.1 |
| Coordinates | 145623..145928 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | H9TJP1 |
| Locus tag | LHL15_RS25580 | Protein ID | WP_000813630.1 |
| Coordinates | 145930..146148 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LHL15_RS25560 (141585) | 141585..142751 | - | 1167 | WP_000772446.1 | plasmid-partitioning protein SopA | - |
| LHL15_RS25565 (143339) | 143339..144094 | - | 756 | WP_000852146.1 | replication initiation protein RepE | - |
| LHL15_RS25570 (144816) | 144816..145622 | - | 807 | WP_000016970.1 | site-specific integrase | - |
| LHL15_RS25575 (145623) | 145623..145928 | - | 306 | WP_001159871.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| LHL15_RS25580 (145930) | 145930..146148 | - | 219 | WP_000813630.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| LHL15_RS25585 (146708) | 146708..146938 | + | 231 | WP_001261286.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| LHL15_RS25590 (146935) | 146935..147351 | + | 417 | WP_001034044.1 | type II toxin-antitoxin system VapC family toxin | - |
| LHL15_RS25595 (147426) | 147426..148991 | + | 1566 | WP_001128474.1 | AAA family ATPase | - |
| LHL15_RS25600 (148976) | 148976..149998 | + | 1023 | WP_000361402.1 | helicase UvrD | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | tet(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD | iutA / iucD / iucC / iucB / iucA | 1..169208 | 169208 | |
| - | flank | IS/Tn | - | - | 150252..150755 | 503 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11692.49 Da Isoelectric Point: 6.4674
>T220144 WP_001159871.1 NZ_CP084902:c145928-145623 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T220144 NZ_CP116962:2817003-2817098 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8354.27 Da Isoelectric Point: 4.5203
>AT220144 WP_000813630.1 NZ_CP084902:c146148-145930 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT220144 NZ_CP116962:c2817198-2817133 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATCAGTAGTAGGGTGTCTTTTTTT
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATCAGTAGTAGGGTGTCTTTTTTT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CCE8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CEF5 |