Detailed information of TA system TA022014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 136927..137567
• Replicon: plasmid 5
• Accession: NC_009350
• Organism: Aeromonas salmonicida subsp. salmonicida A449

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A807Z4U5
• Locus tag: ASA_RS23380
• Protein ID: WP_005320982.1
• Coordinates: 137154..137567 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A807Z5T0
• Locus tag: ASA_RS23375
• Protein ID: WP_005320985.1
• Coordinates: 136927..137157 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASA_RS25230	134595..134957	-	363	WP_005321010.1	hypothetical protein	-
ASA_RS25235	135003..135440	-	438	Protein_149	DNA repair protein RadC	-
ASA_RS23370	136297..136858	+	562	Protein_150	site-specific integrase	-
ASA_RS23375	136927..137157	+	231	WP_005320985.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ASA_RS23380	137154..137567	+	414	WP_005320982.1	PIN domain-containing protein	Toxin
ASA_RS23385	137642..138007	+	366	WP_043145064.1	hypothetical protein	-
ASA_RS23390	138023..138523	+	501	WP_005320977.1	hypothetical protein	-
ASA_RS23395	138545..138853	+	309	WP_005320974.1	hypothetical protein	-
ASA_RS24610	138959..139168	+	210	Protein_156	helix-turn-helix domain-containing protein	-
ASA_RS23400	139390..140931	+	1542	WP_005322071.1	IS21 family transposase	-
ASA_RS23405	140946..141701	+	756	WP_005322068.1	IS21-like element ISAs29 family helper ATPase IstB	-
ASA_RS23410	142238..142390	+	153	WP_005322083.1	Hok/Gef family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-3.1 / mcr-3.15	sycH / aopH / ati2 / ati1 / ascL / ascK / ascJ / ascI / ascH / ascG / ascF / ascE / ascD / ascC / ascB / exsD / exsA / exsB / exsE / exsC / aopD / aopB / acrH / acrV / acrG / acrR / ascV / ascY / ascX / acr2 / acr1 / aopN / ascN / ascO / ascP / ascQ / ascR / ascS / ascT / ascU / aopO	1..155098	155098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-42)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14935.23 Da
• Isoelectric Point: 5.7146

>T22014 WP_005320982.1 NC_009350:137154-137567 [Aeromonas salmonicida subsp. salmonicida A449]
VKTFMLDTCICSFIMREMPQSVLERLGAEVASGNRIVISAITYAEMRYGQIGKKASPKLGPAIDEFVRRLDGILPWDAAT
VDQTLEVRQQLAALGTPIGNNDAAIAAHALTAGCVLVTNNTREFSRVAGLLYEDWVH

Nucleotide

• Length: 414 bp

>T22014 NC_009350:137154-137567 [Aeromonas salmonicida subsp. salmonicida A449]
GTGAAGACGTTTATGCTCGATACCTGCATTTGTTCCTTCATCATGCGCGAAATGCCACAGTCAGTTCTTGAACGGTTGGG
CGCAGAGGTGGCGAGCGGCAATCGGATTGTGATCTCGGCAATCACCTACGCCGAAATGCGTTATGGCCAGATCGGCAAGA
AGGCTTCTCCGAAGCTGGGGCCAGCCATTGATGAATTTGTTCGTCGTCTCGATGGGATCTTGCCATGGGACGCTGCTACG
GTCGATCAGACGCTCGAAGTGCGCCAGCAGTTAGCAGCCTTGGGCACTCCGATCGGCAATAACGATGCTGCTATTGCCGC
CCACGCCCTGACGGCTGGCTGTGTACTGGTAACCAACAATACCCGCGAGTTTTCCCGAGTGGCGGGGCTGCTCTATGAAG
ACTGGGTTCACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8699.88 Da
• Isoelectric Point: 4.7419

>AT22014 WP_005320985.1 NC_009350:136927-137157 [Aeromonas salmonicida subsp. salmonicida A449]
MRTVSIFKNGKNQAVRLPKDMEFQGVNDLEIIKNGDTITLRPARPDWFSLAECQKVDADFLQERTDVISDEGRFAL

Nucleotide

• Length: 231 bp

>AT22014 NC_009350:136927-137157 [Aeromonas salmonicida subsp. salmonicida A449]
ATGCGTACTGTATCAATTTTTAAAAATGGTAAAAATCAGGCGGTGAGACTGCCAAAAGACATGGAGTTTCAGGGGGTTAA
TGACCTTGAGATTATCAAAAATGGCGACACAATCACGTTGCGCCCGGCCCGCCCTGATTGGTTTTCCCTGGCGGAATGCC
AGAAGGTGGATGCAGATTTCTTGCAAGAACGTACCGATGTGATCAGCGATGAAGGACGGTTCGCCCTGTGA

Structures

Toxin

• AlphaFold DB: A0A807Z4U5

Antitoxin

• AlphaFold DB: A0A807Z5T0