Detailed information of TA system TA220136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 84083..85706
• Replicon: plasmid pEC22-CTX-M-15
• Accession: NZ_CP084901
• Organism: Escherichia coli strain EC20

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: LHL15_RS23845
• Protein ID: WP_000088046.1
• Coordinates: 84381..85706 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: LHL15_RS23840
• Protein ID: WP_024136327.1
• Coordinates: 84083..84376 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHL15_RS23815	79955..81196	+	1242	WP_001053338.1	VWA domain-containing protein	-
LHL15_RS25900	81287..81418	+	132	WP_000374059.1	hypothetical protein	-
LHL15_RS23820	81759..82040	-	282	WP_000077926.1	hypothetical protein	-
LHL15_RS23825	82090..82281	-	192	WP_000398480.1	hypothetical protein	-
LHL15_RS23830	82373..82714	-	342	WP_001151575.1	hypothetical protein	-
LHL15_RS23835	83087..83479	+	393	WP_000341066.1	hypothetical protein	-
LHL15_RS23840	84083..84376	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
LHL15_RS23845	84381..85706	+	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
LHL15_RS23850	85767..85973	+	207	WP_000134171.1	hypothetical protein	-
LHL15_RS23855	86075..86485	+	411	WP_000985911.1	hypothetical protein	-
LHL15_RS23860	86498..87313	+	816	WP_053276223.1	HNH endonuclease signature motif containing protein	-
LHL15_RS23865	87597..88294	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
LHL15_RS23870	88338..89003	-	666	WP_000490638.1	hypothetical protein	-
LHL15_RS23875	89061..89441	-	381	WP_001426317.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / qnrB6 / mph(A) / aac(3)-IId / blaCTX-M-15	-	1..231954	231954
	-	flank	IS/Tn	-	-	87597..87872	275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T220136 WP_000088046.1 NZ_CP084901:84381-85706 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T220136 NZ_CP116962:c271447-271304 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT220136 WP_024136327.1 NZ_CP084901:84083-84376 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT220136 NZ_CP116962:271544-271660 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACAAAAATTAATATTTTTGAAATGACCGT

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1