Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3772221..3773056 Replicon chromosome
Accession NZ_CP084900
Organism Escherichia coli strain EC20

Toxin (Protein)


Gene name yeeV Uniprot ID S1EZ92
Locus tag LHL15_RS18355 Protein ID WP_000854726.1
Coordinates 3772221..3772598 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1L6W4Z7
Locus tag LHL15_RS18360 Protein ID WP_070749977.1
Coordinates 3772688..3773056 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHL15_RS18320 (3767348) 3767348..3767653 + 306 WP_000206732.1 protein YfdT -
LHL15_RS18325 (3767653) 3767653..3768003 + 351 WP_000433939.1 helix-turn-helix domain-containing protein -
LHL15_RS18330 (3767925) 3767925..3769043 + 1119 WP_001132557.1 tyrosine-type recombinase/integrase -
LHL15_RS18335 (3769385) 3769385..3770082 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
LHL15_RS18340 (3770099) 3770099..3771295 + 1197 Protein_3593 IS21-like element ISEc12 family transposase -
LHL15_RS18345 (3771310) 3771310..3772056 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
LHL15_RS18350 (3772141) 3772141..3772224 - 84 Protein_3595 DUF5983 family protein -
LHL15_RS18355 (3772221) 3772221..3772598 - 378 WP_000854726.1 TA system toxin CbtA family protein Toxin
LHL15_RS18360 (3772688) 3772688..3773056 - 369 WP_070749977.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LHL15_RS18365 (3773106) 3773106..3773750 - 645 Protein_3598 antitoxin of toxin-antitoxin stability system -
LHL15_RS18370 (3773765) 3773765..3773986 - 222 WP_000692353.1 DUF987 domain-containing protein -
LHL15_RS18375 (3774055) 3774055..3774531 - 477 WP_001186742.1 RadC family protein -
LHL15_RS18380 (3774545) 3774545..3775024 - 480 WP_000706976.1 antirestriction protein -
LHL15_RS18385 (3775118) 3775118..3775363 - 246 WP_000680584.1 hypothetical protein -
LHL15_RS18390 (3775363) 3775363..3776181 - 819 WP_070749978.1 DUF932 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3703737..3779355 75618


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14164.10 Da        Isoelectric Point: 7.8045

>T220131 WP_000854726.1 NZ_CP084900:c3772598-3772221 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Download         Length: 378 bp

>T220131 NZ_CP116957:c1495011-1494742 [Streptococcus pasteurianus]
ATGACTTATAGACTAGTTGTTAGCGATAAGGTCAAGAAACAGCTTAAGAAAATGGATAAGCATGTAAGGCTTATGCTTGC
TAAAGACATGAAAAAGCACCTTGACGGTGTAGAGAATCCCAGACAAATGGGAAAAGCATTAACAGGGCAATTCAAAGGTC
TATGGCGTTACAGAATAGGGAATTACAGGGTTATTTGTGATATTATAGATGATGATTTGGTTATCCTCGCTATCGAAGTA
GGACATAGAAAAGATATTTATAAAAATTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13860.63 Da        Isoelectric Point: 6.3177

>AT220131 WP_070749977.1 NZ_CP084900:c3773056-3772688 [Escherichia coli]
VSDTFHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQVFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGVTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT220131 NZ_CP116957:c1495228-1495001 [Streptococcus pasteurianus]
ATGTCAATCGTATCACTTAGACTAAATAAAAATGAAGAAGAGCTTTTTAAAAGTTATTCTATTCACACAGGAAAAACACT
ATCAGAGTTGTTTAAAACGGCTTTAGCTGAACAAATTGAAGACCAACTAGATTATGAAACAGGTATTAAAGCATTAAATG
AATTTAAAAAGAACCCTGTTACTCACTCTATTGATGACATTATTGAGGAGTTTGAAAATGACTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EZ92

Antitoxin

Source ID Structure
AlphaFold DB A0A1L6W4Z7

References