Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3533405..3534023 Replicon chromosome
Accession NZ_CP084900
Organism Escherichia coli strain EC20

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LHL15_RS17120 Protein ID WP_001291435.1
Coordinates 3533805..3534023 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LHL15_RS17115 Protein ID WP_000344800.1
Coordinates 3533405..3533779 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHL15_RS17105 (3528494) 3528494..3529687 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LHL15_RS17110 (3529710) 3529710..3532859 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LHL15_RS17115 (3533405) 3533405..3533779 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LHL15_RS17120 (3533805) 3533805..3534023 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LHL15_RS17125 (3534195) 3534195..3534746 + 552 WP_000102564.1 maltose O-acetyltransferase -
LHL15_RS17130 (3534862) 3534862..3535332 + 471 WP_000136192.1 YlaC family protein -
LHL15_RS17135 (3535496) 3535496..3537046 + 1551 WP_064221513.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LHL15_RS17140 (3537088) 3537088..3537441 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LHL15_RS17150 (3537820) 3537820..3538131 + 312 WP_000409911.1 MGMT family protein -
LHL15_RS17155 (3538162) 3538162..3538734 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T220130 WP_001291435.1 NZ_CP084900:3533805-3534023 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T220130 NZ_CP116946:57957-58238 [Providencia alcalifaciens]
ATGACTTATAAACTTGAATTTGATAGACGGGCGTTAAAAGAATGGCAAAAACTAGCCCCTCCGATTAGAAATCAATTTAA
AAAGAAACTGTTAGAACGTCTTGAAAATCCTTGTGTTCCATCAGCTAAACTTAGTGGTAAAAATAATCGTTATAAAATAA
AACTACGATCATTAGGTTACCGCTTAATTTATGAAGTGATAGATGAAAAAATTATTCTGTTAGTCATTGCCGTAGGTAAA
AGAGAGGGCAATACTGTCTATTTTTCATCGAACGAACGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT220130 WP_000344800.1 NZ_CP084900:3533405-3533779 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT220130 NZ_CP116946:57716-57967 [Providencia alcalifaciens]
ATGACTTATCAAATTTTAAGTGATACTGCTGCGAGTATTACTGACTTGAAGAAAAATCCAATGGGCACTATTGCAGGTGG
TGAAGGGAACCCCGTAGCTATCCTTAATAGAAATGAACCTGCATTTTATTGTGTTCCCCCTGAACTATTTGCTTGGTTCA
TGGAGTTAGCTGAAGATGCTGAACTTAACAAAATTGCAGATGAACGATTAGAATCACTTGATGTAGTGAGTGTGAACATC
GATGACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References