Detailed information of TA system TA220129

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2872589..2873433
• Replicon: chromosome
• Accession: NZ_CP084900
• Organism: Escherichia coli strain EC20

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: LHL15_RS14015
• Protein ID: WP_000854686.1
• Coordinates: 2872589..2872972 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: LHL15_RS14020
• Protein ID: WP_001285602.1
• Coordinates: 2873053..2873433 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHL15_RS13975 (2867591)	2867591..2868082	-	492	WP_001300785.1	DUF1097 domain-containing protein	-
LHL15_RS13980 (2868184)	2868184..2868738	-	555	WP_001001921.1	molecular chaperone YcdY	-
LHL15_RS13985 (2868762)	2868762..2869499	-	738	WP_000283667.1	zinc-binding phosphatase	-
LHL15_RS13990 (2869554)	2869554..2870492	-	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
LHL15_RS14000 (2870963)	2870963..2871805	-	843	WP_001431817.1	DUF4942 domain-containing protein	-
LHL15_RS14005 (2871890)	2871890..2872087	-	198	WP_000839253.1	DUF957 domain-containing protein	-
LHL15_RS14010 (2872104)	2872104..2872592	-	489	WP_001054233.1	DUF5983 family protein	-
LHL15_RS14015 (2872589)	2872589..2872972	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
LHL15_RS14020 (2873053)	2873053..2873433	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LHL15_RS14025 (2873444)	2873444..2874127	-	684	WP_000086768.1	hypothetical protein	-
LHL15_RS14030 (2874146)	2874146..2874367	-	222	WP_000692298.1	DUF987 domain-containing protein	-
LHL15_RS14035 (2874430)	2874430..2874906	-	477	WP_001186726.1	RadC family protein	-
LHL15_RS14040 (2874922)	2874922..2875407	-	486	WP_000214307.1	antirestriction protein	-
LHL15_RS14045 (2875499)	2875499..2876317	-	819	WP_001234732.1	DUF932 domain-containing protein	-
LHL15_RS14050 (2876417)	2876417..2876650	-	234	WP_001119717.1	DUF905 family protein	-
LHL15_RS14055 (2876729)	2876729..2877184	-	456	WP_001504120.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T220129 WP_000854686.1 NZ_CP084900:c2872972-2872589 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T220129 NZ_CP116946:c19729-19454 [Providencia alcalifaciens]
ATGGAATTAAAATGGACGAGTAAGGCATTATCGGATCTTTCTCGTGTATATGATTTTTTAGCGTTATCAAACTCAGTCGC
TGCCGCGCGTGTTGTGCAAACATTGACGAAAGCCCCCATGTTGTTGTTGATAAAAAACCCCCGAATGGGAGAGCAATTGT
TTCAATTTATCGCTAGAGACTTGGCTGATATGGGGATTAGTGGTCGACGTTTCGCGCTCAATATTGATGTCACTCCAGCG
ACCGCTGGCAATTCGAATTGCTGCTGCATTAGATAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT220129 WP_001285602.1 NZ_CP084900:c2873433-2873053 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT220129 NZ_CP116946:c20010-19720 [Providencia alcalifaciens]
ATGAAAAACAGCTCGGTACAAAATAAGACTAAAGTGCTAACAGCACATGTGCCTTTAACGATGGCAGATAAGATTGATGA
AATAGCCGAACGTCTTGAACGCTCCCGTGGATGGGTCATGAAGCAAGCGTTGTCTACGTGGATAGCTCAAGAGGAGATGC
GGGACACATTGACACGAGAAGCGTTATTGGATGTTGAATCTGGCCAAGTGATAGATAATCAGTCTGTGCAAGATTGGGCG
GCTAGCTTGAATACTGACCTACCATTACCGGTGCCACGCTGATGGAATTAA