Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2414988..2415626 Replicon chromosome
Accession NZ_CP084900
Organism Escherichia coli strain EC20

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag LHL15_RS11650 Protein ID WP_000813794.1
Coordinates 2415450..2415626 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LHL15_RS11645 Protein ID WP_001270286.1
Coordinates 2414988..2415404 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHL15_RS11625 (2410140) 2410140..2411081 - 942 WP_226762403.1 ABC transporter permease -
LHL15_RS11630 (2411082) 2411082..2412095 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
LHL15_RS11635 (2412113) 2412113..2413258 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
LHL15_RS11640 (2413503) 2413503..2414909 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
LHL15_RS11645 (2414988) 2414988..2415404 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LHL15_RS11650 (2415450) 2415450..2415626 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LHL15_RS11655 (2415848) 2415848..2416078 + 231 WP_000494244.1 YncJ family protein -
LHL15_RS11660 (2416170) 2416170..2418131 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LHL15_RS11665 (2418204) 2418204..2418740 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
LHL15_RS11670 (2418832) 2418832..2420007 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T220128 WP_000813794.1 NZ_CP084900:c2415626-2415450 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T220128 NZ_CP116944:c70427-70149 [Providencia alcalifaciens]
ATGATTAAATCGTTTAAACATAAAGGGTTGAAACAACTTTTTGAAAAGGGGAACACATCCGGTGTTCCAGCTCAAGATGC
TGAACGGATCAACGACCGTTTACAAGCAATAGACACCGCTAACGATATTCGTGAATTAGACCGCCAAATTTACAAATTGC
ACCCACTGAAAGGTGACAGAGAAGGTTATTGGTCAATCACAGTGCGAGCGAATTGGCGCATTACTTTTCAATTCATCAAT
GGTGATGCTTACATCTTAAATTATGAGGATTATCACTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT220128 WP_001270286.1 NZ_CP084900:c2415404-2414988 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT220128 NZ_CP116944:c70149-69835 [Providencia alcalifaciens]
ATGAGACAGTTTAAAATATCTCACCCAGGTGAAATTATTGCACGAGATTTGGCTGATATGGGGATCAGTGGTCGACGTTT
TGCATTAAATATTGGTGTAACGCCAGCAACAGTTTCCCGTTTTTTATCAGGTAAAACGGCATTGACTCCCTCATTGGCAA
TCCGTATTGCTGCGGCATTAGGTAGTACGCCTGAATTTTGGTTACGTTTACAAAGTGCTTATGATTTACGCCAATTAGAA
AATAAAATTGATACGTCTGATATTATCTTGTATGAAGAACCATCAGATAGCTCCGAAGGCATATCGAAACATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References