Detailed information of TA system TA022012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelB(antitoxin)
• Location: 1598866..1599478
• Replicon: chromosome
• Accession: NC_009332
• Organism: Streptococcus pyogenes str. Manfredo

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A4Z2JY48
• Locus tag: SPYM_RS08140
• Protein ID: WP_002982731.1
• Coordinates: 1599143..1599478 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q5X9X9
• Locus tag: SPYM_RS08135
• Protein ID: WP_002988079.1
• Coordinates: 1598866..1599153 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPYM_RS08110	1594055..1595038	-	984	WP_011889150.1	tagatose-bisphosphate aldolase	-
SPYM_RS08115	1595042..1595971	-	930	WP_011889151.1	tagatose-6-phosphate kinase	-
SPYM_RS08120	1596019..1596534	-	516	WP_011889152.1	galactose-6-phosphate isomerase subunit LacB	-
SPYM_RS08125	1596569..1596997	-	429	WP_002982747.1	galactose-6-phosphate isomerase subunit LacA	-
SPYM_RS08130	1597443..1598216	+	774	WP_002982738.1	DeoR/GlpR family DNA-binding transcription regulator	-
SPYM_RS08135	1598866..1599153	+	288	WP_002988079.1	DNA-damage-inducible protein J	Antitoxin
SPYM_RS08140	1599143..1599478	+	336	WP_002982731.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SPYM_RS09925	1599630..1600100	+	471	WP_021733374.1	site-specific integrase	-
SPYM_RS09930	1600063..1600611	+	549	WP_136115776.1	site-specific integrase	-
SPYM_RS08150	1600731..1601123	-	393	WP_002982716.1	30S ribosomal protein S9	-
SPYM_RS08155	1601144..1601590	-	447	WP_002982710.1	50S ribosomal protein L13	-
SPYM_RS08160	1601808..1602014	-	207	WP_002982695.1	helix-turn-helix transcriptional regulator	-
SPYM_RS08165	1602011..1602517	-	507	WP_002988070.1	hypothetical protein	-
SPYM_RS08170	1602653..1603513	-	861	WP_011889153.1	DegV family protein	-
SPYM_RS08175	1603610..1604128	-	519	WP_011889154.1	NYN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-105)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 13145.88 Da
• Isoelectric Point: 5.2144

>T22012 WP_002982731.1 NC_009332:1599143-1599478 [Streptococcus pyogenes str. Manfredo]
MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISDIEKLEVFPEVGFDADEKYGSKISHYHSTKGYTLSKD
YIVLYHIEGEENRVVIDYLLPTQSDYIKLFK

Nucleotide

• Length: 336 bp

>T22012 NC_009332:1599143-1599478 [Streptococcus pyogenes str. Manfredo]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCAGCCATTATCACAGCACTAAAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGGTCGTTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10934.56 Da
• Isoelectric Point: 5.2318

>AT22012 WP_002988079.1 NC_009332:1598866-1599153 [Streptococcus pyogenes str. Manfredo]
MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNVVVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQ
GKFYTSEEVRAELGL

Nucleotide

• Length: 288 bp

>AT22012 NC_009332:1598866-1599153 [Streptococcus pyogenes str. Manfredo]
ATGACTACAGTAAAAAAAAACAGAGCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Structures

Toxin

• AlphaFold DB: A0A4Z2JY48

Antitoxin

• AlphaFold DB: A0A4U7GX94