Detailed information of TA system TA022010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3140575..3141208
• Replicon: chromosome
• Accession: NC_009253
• Organism: Desulfotomaculum reducens MI-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A4J8J5
• Locus tag: DRED_RS15410
• Protein ID: WP_011879191.1
• Coordinates: 3140575..3140925 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A4J8J6
• Locus tag: DRED_RS15415
• Protein ID: WP_011879192.1
• Coordinates: 3140933..3141208 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRED_RS15390	3136512..3137810	-	1299	WP_011879187.1	phosphomethylpyrimidine synthase ThiC	-
DRED_RS15395	3138094..3138501	-	408	WP_011879188.1	response regulator	-
DRED_RS15400	3138629..3139786	-	1158	WP_011879189.1	amidohydrolase	-
DRED_RS15405	3139814..3140515	-	702	WP_011879190.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
DRED_RS15410	3140575..3140925	-	351	WP_011879191.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DRED_RS15415	3140933..3141208	-	276	WP_011879192.1	ribbon-helix-helix protein, CopG family	Antitoxin
DRED_RS15420	3141273..3141788	-	516	WP_011879193.1	acetolactate synthase small subunit	-
DRED_RS15425	3141919..3142293	-	375	WP_011879194.1	zinc ribbon domain-containing protein	-
DRED_RS15430	3142310..3142765	-	456	WP_011879195.1	N-acetyltransferase	-
DRED_RS18405	3142952..3143287	+	336	WP_011879196.1	hypothetical protein	-
DRED_RS15435	3143329..3143976	-	648	WP_011879197.1	rhomboid family intramembrane serine protease	-
DRED_RS15440	3144098..3144430	-	333	WP_011879198.1	hypothetical protein	-
DRED_RS15445	3144539..3145972	-	1434	WP_011879199.1	glucose-6-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (8-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12778.71 Da
• Isoelectric Point: 5.8579

>T22010 WP_011879191.1 NC_009253:c3140925-3140575 [Desulfotomaculum reducens MI-1]
MQVRRGDVFYADLSPVVGSEQGGIRPVLILQNDIGNQYSPTTIIAAITSQIAKAKLPTHVEIQAKESGLEKDSVVLLEQL
RTIDKSRLFEKVSSLNNDFMNKVNRAVEISLGLVEI

Nucleotide

• Length: 351 bp

>T22010 NC_009253:c3140925-3140575 [Desulfotomaculum reducens MI-1]
ATGCAAGTCCGGCGAGGAGATGTTTTTTACGCTGACCTCAGCCCTGTGGTCGGCTCAGAACAGGGAGGCATCAGGCCGGT
GTTGATTCTGCAAAACGATATTGGGAATCAGTACAGTCCCACTACCATTATTGCTGCCATAACCTCCCAAATTGCAAAGG
CAAAGTTACCTACTCACGTTGAAATCCAGGCCAAGGAAAGTGGGCTTGAGAAAGATTCTGTGGTTCTTTTGGAACAGCTT
AGAACCATTGATAAAAGTAGGCTGTTTGAGAAGGTATCCTCTTTAAACAATGATTTCATGAACAAAGTGAACCGGGCCGT
TGAAATTAGTCTGGGACTGGTGGAAATCTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10657.37 Da
• Isoelectric Point: 6.4089

>AT22010 WP_011879192.1 NC_009253:c3141208-3140933 [Desulfotomaculum reducens MI-1]
VSQAQIKRIMISLPDSLLEEVDNIVEEERVNRSEFIREAMKLYIAERKRRLLREQMKKGYLEMAKLNLALAIEYQRIENV
SSGYELAKAEG

Nucleotide

• Length: 276 bp

>AT22010 NC_009253:c3141208-3140933 [Desulfotomaculum reducens MI-1]
GTGTCACAAGCACAGATTAAACGAATCATGATTAGCCTACCCGACAGTCTGCTGGAGGAAGTGGACAATATCGTCGAGGA
AGAAAGGGTCAATCGCAGTGAGTTTATTAGGGAGGCAATGAAGTTATATATTGCTGAGCGTAAACGACGCCTTTTAAGGG
AACAAATGAAAAAAGGTTATTTAGAAATGGCTAAATTAAACCTGGCTCTGGCCATCGAATACCAGCGCATTGAAAATGTT
TCCAGTGGTTACGAGCTGGCCAAAGCGGAGGGATAA

Structures

Toxin

• AlphaFold DB: A4J8J5

Antitoxin

• AlphaFold DB: A4J8J6