Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2783190..2783857 Replicon chromosome
Accession NZ_CP084899
Organism Escherichia coli strain W3110-P

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag LHV60_RS13660 Protein ID WP_001094400.1
Coordinates 2783528..2783857 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag LHV60_RS13655 Protein ID WP_000072690.1
Coordinates 2783190..2783507 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV60_RS13625 (2778242) 2778242..2779251 - 1010 Protein_2665 arsenic transporter -
LHV60_RS13630 (2779393) 2779393..2781096 + 1704 WP_000896263.1 protein YfjW -
LHV60_RS13635 (2781668) 2781668..2781891 + 224 Protein_2667 DUF905 domain-containing protein -
LHV60_RS13640 (2781994) 2781994..2782452 + 459 WP_000211841.1 antirestriction protein -
LHV60_RS13645 (2782461) 2782461..2782943 + 483 WP_001407480.1 RadC family protein -
LHV60_RS13650 (2782952) 2782952..2783152 + 201 WP_001395452.1 DUF987 domain-containing protein -
LHV60_RS13655 (2783190) 2783190..2783507 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LHV60_RS13660 (2783528) 2783528..2783857 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
LHV60_RS13665 (2784221) 2784221..2788801 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T220097 WP_001094400.1 NZ_CP084899:2783528-2783857 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T220097 NZ_CP116929:c4115463-4115290 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT220097 WP_000072690.1 NZ_CP084899:2783190-2783507 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT220097 NZ_CP116929:c4115787-4115491 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGGCTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References