Detailed information of TA system TA022009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1448554..1449118
• Replicon: chromosome
• Accession: NC_009142
• Organism: Saccharopolyspora erythraea NRRL 2338

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A4F9B1
• Locus tag: SACE_RS06420
• Protein ID: WP_009947934.1
• Coordinates: 1448554..1448901 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A4F9B2
• Locus tag: SACE_RS06425
• Protein ID: WP_009947932.1
• Coordinates: 1448888..1449118 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SACE_RS06395	1443695..1444267	+	573	WP_009947940.1	siderophore-interacting protein	-
SACE_RS06400	1444382..1445722	+	1341	WP_021341391.1	lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein	-
SACE_RS06405	1445857..1446915	+	1059	WP_009947937.1	LLM class flavin-dependent oxidoreductase	-
SACE_RS06410	1447034..1447423	+	390	WP_011873324.1	DUF3817 domain-containing protein	-
SACE_RS06415	1447420..1448358	+	939	WP_044547166.1	heme o synthase	-
SACE_RS06420	1448554..1448901	-	348	WP_009947934.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SACE_RS06425	1448888..1449118	-	231	WP_009947932.1	antitoxin	Antitoxin
SACE_RS06430	1449239..1449865	-	627	WP_009947931.1	maleylpyruvate isomerase N-terminal domain-containing protein	-
SACE_RS06435	1450085..1450636	+	552	WP_009947930.1	snapalysin family zinc-dependent metalloprotease	-
SACE_RS06440	1450838..1452604	+	1767	WP_009947928.1	alpha-L-fucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12642.51 Da
• Isoelectric Point: 11.5554

>T22009 WP_009947934.1 NC_009142:c1448901-1448554 [Saccharopolyspora erythraea NRRL 2338]
MRRGDVYWVDFEPIRGSEADKTRPAVVVSNDAANRSAERARRGVITVVPLTSNVNRVLPFQVRLRAADCGLAGDSKAQAE
QVRAVAFHRLGKRIGRVPDTTLAKLDNALRLHLGL

Nucleotide

• Length: 348 bp

>T22009 NC_009142:c1448901-1448554 [Saccharopolyspora erythraea NRRL 2338]
ATGCGTCGAGGTGACGTCTACTGGGTCGACTTCGAACCCATCCGCGGCTCGGAGGCCGACAAGACCCGCCCGGCCGTCGT
GGTCAGCAACGACGCCGCCAACCGCAGCGCGGAGCGCGCCCGGCGCGGTGTGATCACCGTCGTGCCCCTGACTTCCAACG
TGAACCGCGTGCTGCCGTTCCAGGTCCGGCTGCGCGCTGCCGACTGCGGCCTGGCCGGCGACTCGAAGGCGCAGGCCGAG
CAGGTGCGGGCGGTGGCGTTCCACCGCCTCGGCAAGCGCATCGGCCGGGTGCCTGACACCACCCTCGCCAAGCTCGACAA
CGCCCTGCGGCTGCATTTGGGGCTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8319.04 Da
• Isoelectric Point: 3.9693

>AT22009 WP_009947932.1 NC_009142:c1449118-1448888 [Saccharopolyspora erythraea NRRL 2338]
MKLSVSLTDEDVTFVDEYAQQVGAPSRSAVIHQAISLLRSAELEDAYASAWAEWESGDDRKLWETTASDGIADASR

Nucleotide

• Length: 231 bp

>AT22009 NC_009142:c1449118-1448888 [Saccharopolyspora erythraea NRRL 2338]
ATGAAACTCAGTGTGAGCCTCACCGACGAAGACGTGACGTTCGTCGACGAGTACGCCCAGCAGGTGGGGGCGCCGTCCAG
ATCCGCCGTGATCCACCAGGCCATCAGCCTGCTGCGGAGCGCCGAGCTGGAAGATGCCTACGCCAGCGCGTGGGCGGAGT
GGGAAAGCGGCGATGACCGGAAGCTGTGGGAGACGACCGCCTCGGACGGCATCGCAGATGCGTCGAGGTGA

Structures

Toxin

• AlphaFold DB: A4F9B1

Antitoxin

• AlphaFold DB: A4F9B2