220071

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 262552..263231 Replicon chromosome
Accession NZ_CP084899
Organism Escherichia coli strain W3110-P

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag LHV60_RS01245 Protein ID WP_000854672.1
Coordinates 262552..262893 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag LHV60_RS01250 Protein ID WP_000070395.1
Coordinates 262914..263231 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV60_RS01220 (257829) 257829..258230 + 402 WP_000174689.1 sigma factor-binding protein Crl -
LHV60_RS01225 (258269) 258269..259324 - 1056 WP_000749863.1 phosphoporin PhoE -
LHV60_RS01230 (259612) 259612..260715 + 1104 WP_001285288.1 glutamate 5-kinase -
LHV60_RS01235 (260727) 260727..261980 + 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
LHV60_RS01245 (262552) 262552..262893 - 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
LHV60_RS01250 (262914) 262914..263231 - 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
LHV60_RS01255 (263250) 263250..263471 - 222 WP_000691994.1 DUF987 domain-containing protein -
LHV60_RS01260 (263480) 263480..263956 - 477 WP_000811693.1 RadC family protein -
LHV60_RS01265 (263972) 263972..264430 - 459 WP_000211838.1 antirestriction protein -
LHV60_RS01270 (264528) 264528..264767 - 240 WP_000194654.1 DUF905 family protein -
LHV60_RS01275 (264844) 264844..265311 - 468 WP_001547765.1 protein YkfB -
LHV60_RS01280 (265334) 265334..265777 - 444 WP_000824223.1 lipoprotein YafY -
LHV60_RS01285 (265777) 265777..265953 - 177 WP_001285112.1 hypothetical protein -
LHV60_RS01290 (266000) 266000..266191 - 192 Protein_249 DeoR family transcriptional regulator -
LHV60_RS01295 (266408) 266408..267229 - 822 WP_000197389.1 DUF932 domain-containing protein -
LHV60_RS01300 (267321) 267321..268184 - 864 WP_001065553.1 GTPase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T220071 WP_000854672.1 NZ_CP084899:c262893-262552 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T220071 NZ_CP116925:2759667-2759774 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT220071 WP_000070395.1 NZ_CP084899:c263231-262914 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT220071 NZ_CP116925:c2759620-2759554 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P77692

Antitoxin

Source ID Structure
AlphaFold DB Q47684

References