Detailed information of TA system TA220064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4542769..4543583
• Replicon: chromosome
• Accession: NZ_CP084898
• Organism: Escherichia coli strain gw25

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: LHV65_RS22075
• Protein ID: WP_001054376.1
• Coordinates: 4543326..4543583 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: LHV65_RS22070
• Protein ID: WP_001309181.1
• Coordinates: 4542769..4543314 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHV65_RS22040 (4538460)	4538460..4539773	-	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-
LHV65_RS22045 (4539785)	4539785..4540063	-	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
LHV65_RS22050 (4540060)	4540060..4541181	-	1122	WP_000010829.1	M42 family metallopeptidase	-
LHV65_RS22055 (4541426)	4541426..4541542	-	117	Protein_4293	VOC family protein	-
LHV65_RS22060 (4541580)	4541580..4541798	-	219	Protein_4294	hypothetical protein	-
LHV65_RS22065 (4541967)	4541967..4542713	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
LHV65_RS22070 (4542769)	4542769..4543314	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
LHV65_RS22075 (4543326)	4543326..4543583	-	258	WP_001054376.1	YjhX family toxin	Toxin
LHV65_RS22080 (4544074)	4544074..4544205	-	132	WP_001309182.1	hypothetical protein	-
LHV65_RS22085 (4544321)	4544321..4545561	+	1241	Protein_4299	helicase YjhR	-
LHV65_RS22090 (4545829)	4545829..4546034	-	206	Protein_4300	HNH endonuclease	-
LHV65_RS22095 (4546144)	4546144..4547124	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
LHV65_RS22100 (4547189)	4547189..4548295	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T220064 WP_001054376.1 NZ_CP084898:c4543583-4543326 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T220064 NZ_CP116925:1800554-1800808 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT220064 WP_001309181.1 NZ_CP084898:c4543314-4542769 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT220064 NZ_CP116925:1800306-1800557 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8