Detailed information of TA system TA022005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 535900..537440
• Replicon: chromosome
• Accession: NC_009092
• Organism: Shewanella loihica PV-4

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A3QA53
• Locus tag: SHEW_RS02460
• Protein ID: WP_011864285.1
• Coordinates: 536139..537440 (+)
• Length: 434 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: SHEW_RS02455
• Protein ID: WP_011864284.1
• Coordinates: 535900..536136 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHEW_RS02430	531629..532156	-	528	WP_011864279.1	hypothetical protein	-
SHEW_RS02435	532153..532500	-	348	WP_011864280.1	STAS-like domain-containing protein	-
SHEW_RS02440	532487..533653	-	1167	WP_011864281.1	hypothetical protein	-
SHEW_RS02445	533870..534667	-	798	WP_011864282.1	NYN domain-containing protein	-
SHEW_RS02450	534699..535073	-	375	WP_041406321.1	hypothetical protein	-
SHEW_RS02455	535900..536136	+	237	WP_011864284.1	helix-turn-helix domain-containing protein	Antitoxin
SHEW_RS02460	536139..537440	+	1302	WP_011864285.1	HipA domain-containing protein	Toxin
SHEW_RS02465	537528..538568	+	1041	WP_041406323.1	sel1 repeat family protein	-
SHEW_RS02470	538703..539107	+	405	WP_011864287.1	hypothetical protein	-
SHEW_RS02475	539397..540563	+	1167	WP_011864288.1	BamA/TamA family outer membrane protein	-
SHEW_RS02480	540704..541378	+	675	WP_041406907.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 434 a.a.
• Molecular weight: 48421.39 Da
• Isoelectric Point: 4.6059

>T22005 WP_011864285.1 NC_009092:536139-537440 [Shewanella loihica PV-4]
MAESPILVLDMLLGDQLIGALSFDPSDERFAVSYTQAWQQSGFPLSPTIPLDGSGSSTQIAMFLVNLLPENKGLDYLVES
LGVAKGNTFALIRAIGLDTAGAIAFSSKGSAFPPTTLRTISDDEIVQRLEDPEFWPMEIWDGRPRLSVAGVQSKLNLFFN
GEEFGFGEGALSSTHILKFEQHPHLVINEFLTMRLAKALALKVANVEIRCFGQYKSLCVERFDRRYLDDENRVQRRHIID
SCQALGFSVNKKYERNFGSGRDVRDIREGVSLPRLFGLANQCINPAAAKQDMLRWTLFNLLSGNADAHGKNYSFFMTPNG
MLPTPWYDLVCVALYEDFEQELAMAIDDEFDPNKIYAYQLAAFSEEVGLPRALIVNSLDKMVAKIRLEINGVIAMLKDLD
EDEQAFVEAYKAQLLARCERYSAIAPEIKAIEL

Nucleotide

• Length: 1302 bp

>T22005 NC_009092:536139-537440 [Shewanella loihica PV-4]
ATGGCCGAATCACCAATCCTTGTGTTAGATATGCTACTTGGTGACCAGCTTATCGGCGCGTTGTCATTTGATCCAAGCGA
TGAGCGTTTTGCGGTCAGCTACACTCAAGCCTGGCAGCAAAGCGGTTTTCCATTATCACCTACGATCCCTTTGGATGGCT
CGGGTAGCAGTACGCAAATTGCCATGTTTCTGGTTAATTTGCTGCCTGAGAATAAGGGGCTCGATTACCTTGTTGAATCG
CTTGGTGTTGCAAAGGGCAATACCTTTGCGCTCATCCGGGCCATTGGCCTAGATACTGCGGGTGCCATCGCCTTTTCTTC
TAAAGGTTCTGCATTTCCACCGACAACACTCCGGACTATCAGTGACGATGAGATCGTTCAGCGACTGGAAGATCCTGAGT
TCTGGCCGATGGAGATTTGGGATGGTAGACCCAGGCTGTCGGTGGCTGGGGTACAGTCAAAGTTAAATCTCTTCTTTAAT
GGTGAGGAGTTTGGCTTCGGTGAAGGAGCACTGTCATCGACACATATCCTGAAGTTTGAACAGCATCCGCATCTGGTCAT
CAACGAGTTCCTTACTATGCGCTTGGCTAAGGCGCTGGCCTTGAAAGTCGCGAATGTCGAAATTCGTTGTTTTGGCCAGT
ATAAATCCTTGTGTGTCGAACGTTTCGATCGTCGCTATCTGGACGATGAAAACAGGGTGCAGAGGCGGCACATTATCGAT
AGTTGCCAAGCACTTGGTTTTAGCGTCAACAAGAAATATGAACGTAACTTTGGTAGTGGACGGGATGTCAGGGATATTCG
TGAAGGTGTCAGCCTGCCAAGGTTGTTTGGTCTTGCGAATCAATGTATTAATCCGGCTGCAGCCAAGCAGGATATGTTGC
GGTGGACGCTGTTCAATTTATTGAGTGGCAATGCCGATGCCCATGGGAAAAACTACTCGTTTTTTATGACGCCTAACGGG
ATGTTACCTACACCTTGGTATGACCTCGTTTGCGTGGCCTTATATGAGGATTTTGAACAAGAGTTGGCAATGGCGATAGA
TGATGAGTTCGATCCCAATAAGATCTATGCCTATCAACTTGCCGCCTTTAGTGAAGAGGTGGGATTACCAAGAGCCTTAA
TCGTAAATAGCCTGGATAAGATGGTGGCTAAGATAAGGCTCGAAATTAATGGTGTCATCGCCATGCTCAAAGACTTGGAC
GAAGATGAGCAGGCTTTTGTGGAGGCATATAAGGCACAACTGCTGGCCAGATGCGAACGTTATTCGGCGATCGCTCCTGA
AATCAAGGCTATTGAGCTTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8520.86 Da
• Isoelectric Point: 7.9713

>AT22005 WP_011864284.1 NC_009092:535900-536136 [Shewanella loihica PV-4]
MSSSLNQDSLGLLIKTRRKEAALTQEVAAMLCGVTKKTLIRVEKGEDVYISTVFKILDGLGIRLAVESKSDAGSSEWY

Nucleotide

• Length: 237 bp

>AT22005 NC_009092:535900-536136 [Shewanella loihica PV-4]
ATGAGCTCCTCGTTAAACCAAGATTCGTTGGGATTACTGATTAAAACCCGCAGGAAGGAAGCGGCGCTGACTCAAGAGGT
GGCGGCAATGTTGTGCGGTGTCACTAAGAAAACCTTGATTCGTGTCGAGAAGGGCGAAGATGTCTACATCTCTACCGTAT
TCAAGATACTTGATGGTCTGGGGATTCGGCTCGCTGTCGAGTCAAAGTCAGATGCCGGCTCAAGTGAGTGGTACTAG

Structures

Toxin

• AlphaFold DB: A3QA53