Detailed information of TA system TA220040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1511610..1512248
• Replicon: chromosome
• Accession: NZ_CP084898
• Organism: Escherichia coli strain gw25

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LHV65_RS07435
• Protein ID: WP_000813794.1
• Coordinates: 1511610..1511786 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LHV65_RS07440
• Protein ID: WP_001270286.1
• Coordinates: 1511832..1512248 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHV65_RS07415 (1507229)	1507229..1508443	-	1215	WP_001326689.1	BenE family transporter YdcO	-
LHV65_RS07420 (1508496)	1508496..1509032	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LHV65_RS07425 (1509105)	1509105..1511066	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LHV65_RS07430 (1511158)	1511158..1511388	-	231	WP_000494244.1	YncJ family protein	-
LHV65_RS07435 (1511610)	1511610..1511786	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LHV65_RS07440 (1511832)	1511832..1512248	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LHV65_RS07445 (1512327)	1512327..1513733	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
LHV65_RS07450 (1513978)	1513978..1515123	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
LHV65_RS07455 (1515141)	1515141..1516154	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LHV65_RS07460 (1516155)	1516155..1517096	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T220040 WP_000813794.1 NZ_CP084898:1511610-1511786 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T220040 NZ_CP116923:1774291-1774542 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT220040 WP_001270286.1 NZ_CP084898:1511832-1512248 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT220040 NZ_CP116923:1774539-1774793 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• PDB: 6HPB