Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3046491..3047145 Replicon chromosome
Accession NZ_CP084897
Organism Escherichia coli strain gb37

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LHV64_RS14875 Protein ID WP_000244777.1
Coordinates 3046491..3046898 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LHV64_RS14880 Protein ID WP_000354046.1
Coordinates 3046879..3047145 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV64_RS14855 (3042448) 3042448..3044181 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
LHV64_RS14860 (3044187) 3044187..3044897 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LHV64_RS14865 (3044922) 3044922..3045818 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LHV64_RS14870 (3045930) 3045930..3046451 + 522 WP_001055874.1 flavodoxin FldB -
LHV64_RS14875 (3046491) 3046491..3046898 - 408 WP_000244777.1 protein YgfX Toxin
LHV64_RS14880 (3046879) 3046879..3047145 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LHV64_RS14885 (3047388) 3047388..3048368 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LHV64_RS14890 (3048564) 3048564..3049223 - 660 WP_000250274.1 hemolysin III family protein -
LHV64_RS14895 (3049387) 3049387..3049698 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LHV64_RS14900 (3049743) 3049743..3051176 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
LHV64_RS14905 (3051233) 3051233..3051976 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T220013 WP_000244777.1 NZ_CP084897:c3046898-3046491 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T220013 NZ_CP116916:3399017-3399235 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT220013 WP_000354046.1 NZ_CP084897:c3047145-3046879 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT220013 NZ_CP116916:3398617-3398991 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References