Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2082079..2082910 Replicon chromosome
Accession NZ_CP084896
Organism Escherichia coli strain g62

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag LHV62_RS10355 Protein ID WP_000854814.1
Coordinates 2082536..2082910 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag LHV62_RS10350 Protein ID WP_001285584.1
Coordinates 2082079..2082447 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV62_RS10335 (2079746) 2079746..2081278 + 1533 WP_001350525.1 protein YeeR -
LHV62_RS10340 (2081275) 2081275..2081721 + 447 WP_000187523.1 RadC family protein -
LHV62_RS10345 (2081784) 2081784..2082005 + 222 WP_000692323.1 DUF987 domain-containing protein -
LHV62_RS10350 (2082079) 2082079..2082447 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
LHV62_RS10355 (2082536) 2082536..2082910 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
LHV62_RS10360 (2082907) 2082907..2083101 + 195 WP_000988600.1 DUF5983 family protein -
LHV62_RS10365 (2083147) 2083147..2083227 + 81 Protein_2025 hypothetical protein -
LHV62_RS10370 (2083516) 2083516..2083644 - 129 Protein_2026 transposase domain-containing protein -
LHV62_RS10375 (2083764) 2083764..2083898 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
LHV62_RS10380 (2083999) 2083999..2084328 - 330 WP_000450409.1 DUF496 family protein -
LHV62_RS10385 (2084500) 2084500..2085558 - 1059 WP_001200891.1 FUSC family protein -
LHV62_RS10390 (2085756) 2085756..2086229 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
LHV62_RS10395 (2086348) 2086348..2087514 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T219965 WP_000854814.1 NZ_CP084896:2082536-2082910 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T219965 NZ_CP116904:c52836-52525 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT219965 WP_001285584.1 NZ_CP084896:2082079-2082447 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT219965 NZ_CP116904:c52528-52109 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References