Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4457978..4458573 Replicon chromosome
Accession NZ_CP084895
Organism Escherichia coli strain g74

Toxin (Protein)


Gene name chpB Uniprot ID U9XNP6
Locus tag LHV63_RS21645 Protein ID WP_000239577.1
Coordinates 4458223..4458573 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag LHV63_RS21640 Protein ID WP_001223208.1
Coordinates 4457978..4458229 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV63_RS21630 (4453643) 4453643..4457422 + 3780 WP_000060911.1 autotransporter assembly complex protein TamB -
LHV63_RS21635 (4457425) 4457425..4457766 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
LHV63_RS21640 (4457978) 4457978..4458229 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
LHV63_RS21645 (4458223) 4458223..4458573 + 351 WP_000239577.1 endoribonuclease toxin ChpB Toxin
LHV63_RS21650 (4458653) 4458653..4459183 - 531 WP_000055075.1 inorganic diphosphatase -
LHV63_RS21655 (4459493) 4459493..4460449 + 957 WP_000265913.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
LHV63_RS21660 (4460589) 4460589..4462091 + 1503 WP_000205805.1 sugar ABC transporter ATP-binding protein -
LHV63_RS21665 (4462105) 4462105..4463127 + 1023 WP_001313531.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12492.40 Da        Isoelectric Point: 5.5572

>T219934 WP_000239577.1 NZ_CP084895:4458223-4458573 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T219934 NZ_CP116900:2170714-2171010 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT219934 WP_001223208.1 NZ_CP084895:4457978-4458229 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT219934 NZ_CP116900:2171012-2171407 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XNP6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References