Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2771994..2773431 Replicon chromosome
Accession NZ_CP084895
Organism Escherichia coli strain g74

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag LHV63_RS13580 Protein ID WP_000155570.1
Coordinates 2771994..2773067 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag LHV63_RS13585 Protein ID WP_000461704.1
Coordinates 2773060..2773431 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV63_RS13565 (2767427) 2767427..2769616 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
LHV63_RS13570 (2769613) 2769613..2771229 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
LHV63_RS13575 (2771589) 2771589..2771852 - 264 WP_000502050.1 DUF1524 domain-containing protein -
LHV63_RS13580 (2771994) 2771994..2773067 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
LHV63_RS13585 (2773060) 2773060..2773431 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
LHV63_RS13590 (2773786) 2773786..2774649 + 864 WP_000787597.1 GTPase family protein -
LHV63_RS13595 (2774741) 2774741..2775562 + 822 WP_000197391.1 DUF932 domain-containing protein -
LHV63_RS13600 (2775779) 2775779..2776480 + 702 WP_000189409.1 WYL domain-containing protein -
LHV63_RS13605 (2776521) 2776521..2776757 + 237 WP_001144029.1 protein YpjK -
LHV63_RS13610 (2776757) 2776757..2777200 + 444 WP_000824222.1 lipoprotein YfjS -
LHV63_RS13615 (2777224) 2777224..2777691 + 468 WP_000702495.1 protein YfjT -
LHV63_RS13620 (2778078) 2778078..2778230 - 153 Protein_2664 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T219924 WP_000155570.1 NZ_CP084895:2771994-2773067 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T219924 NZ_CP116892:240444-240794 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT219924 WP_000461704.1 NZ_CP084895:2773060-2773431 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT219924 NZ_CP116892:240152-240439 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References