Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2082080..2082911 Replicon chromosome
Accession NZ_CP084895
Organism Escherichia coli strain g74

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag LHV63_RS10355 Protein ID WP_000854814.1
Coordinates 2082537..2082911 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag LHV63_RS10350 Protein ID WP_001285584.1
Coordinates 2082080..2082448 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV63_RS10335 (2079747) 2079747..2081279 + 1533 WP_001350525.1 protein YeeR -
LHV63_RS10340 (2081276) 2081276..2081722 + 447 WP_000187523.1 RadC family protein -
LHV63_RS10345 (2081785) 2081785..2082006 + 222 WP_000692323.1 DUF987 domain-containing protein -
LHV63_RS10350 (2082080) 2082080..2082448 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
LHV63_RS10355 (2082537) 2082537..2082911 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
LHV63_RS10360 (2082908) 2082908..2083102 + 195 WP_000988600.1 DUF5983 family protein -
LHV63_RS10365 (2083148) 2083148..2083228 + 81 Protein_2025 hypothetical protein -
LHV63_RS10370 (2083517) 2083517..2083645 - 129 Protein_2026 transposase domain-containing protein -
LHV63_RS10375 (2083765) 2083765..2083899 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
LHV63_RS10380 (2084000) 2084000..2084329 - 330 WP_000450409.1 DUF496 family protein -
LHV63_RS10385 (2084501) 2084501..2085559 - 1059 WP_001200891.1 FUSC family protein -
LHV63_RS10390 (2085757) 2085757..2086230 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
LHV63_RS10395 (2086349) 2086349..2087515 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T219922 WP_000854814.1 NZ_CP084895:2082537-2082911 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T219922 NZ_CP116889:240482-240832 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT219922 WP_001285584.1 NZ_CP084895:2082080-2082448 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT219922 NZ_CP116889:240190-240477 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References