Detailed information of TA system TA219892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4542770..4543584
• Replicon: chromosome
• Accession: NZ_CP084894
• Organism: Escherichia coli strain g83

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: LHV61_RS22075
• Protein ID: WP_001054376.1
• Coordinates: 4543327..4543584 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: LHV61_RS22070
• Protein ID: WP_001309181.1
• Coordinates: 4542770..4543315 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHV61_RS22040 (4538461)	4538461..4539774	-	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-
LHV61_RS22045 (4539786)	4539786..4540064	-	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
LHV61_RS22050 (4540061)	4540061..4541182	-	1122	WP_000010829.1	M42 family metallopeptidase	-
LHV61_RS22055 (4541427)	4541427..4541543	-	117	Protein_4293	VOC family protein	-
LHV61_RS22060 (4541581)	4541581..4541799	-	219	Protein_4294	hypothetical protein	-
LHV61_RS22065 (4541968)	4541968..4542714	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
LHV61_RS22070 (4542770)	4542770..4543315	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
LHV61_RS22075 (4543327)	4543327..4543584	-	258	WP_001054376.1	YjhX family toxin	Toxin
LHV61_RS22080 (4544075)	4544075..4544206	-	132	WP_001309182.1	hypothetical protein	-
LHV61_RS22085 (4544322)	4544322..4545562	+	1241	Protein_4299	helicase YjhR	-
LHV61_RS22090 (4545830)	4545830..4546035	-	206	Protein_4300	HNH endonuclease	-
LHV61_RS22095 (4546145)	4546145..4547125	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
LHV61_RS22100 (4547190)	4547190..4548296	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T219892 WP_001054376.1 NZ_CP084894:c4543584-4543327 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T219892 NZ_CP116807:c1050137-1049766 [Mammaliicoccus lentus]
ATGAGAAGAGGAGATGTATATCTCGCAGATCTTTCTCCAGTAACTGGTTCTGAACAAGGGGGAACAAGACCAGTCGTTAT
TATACAAAATGACACAGGTAATAGATATAGTCCGACTGTTATTGTAGCAGCGATTACTGGGAAGATTAATAAAGCTAAAA
TACCTACACATGTAGAAATTGAAGCAGCGAAATATAAACTAGATAGAGATTCAGTTATTTTGCTAGAACAAATTAGAACA
ATCGATAAGAAACGGTTGAAAGAAAAATTAACATATTTATCTGATGCAAAGATGAAAGAGGTTGATTCTGCAATTGCAAT
TAGCCTCAATCTCACTTTGCAATATAAATTTGATACATTAGGTAATACATAG

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT219892 WP_001309181.1 NZ_CP084894:c4543315-4542770 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT219892 NZ_CP116807:c1050305-1050138 [Mammaliicoccus lentus]
ATGACATCGTTAACACAAAATCGCAATCAAAGTTTAGAACAAACACTGAAAGAAGGATACCGTTATATGGCTGATTTAAA
TCTCTCCCTTGCGTCTGAAGCATATTATAGTGAATGTGAAGCTTGTGATTCTAATGAGTCACACTTAACTTCTCAACAAG
GTGAATAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8