Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2783191..2783858 Replicon chromosome
Accession NZ_CP084894
Organism Escherichia coli strain g83

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag LHV61_RS13660 Protein ID WP_001094400.1
Coordinates 2783529..2783858 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag LHV61_RS13655 Protein ID WP_000072690.1
Coordinates 2783191..2783508 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV61_RS13625 (2778243) 2778243..2779252 - 1010 Protein_2665 arsenic transporter -
LHV61_RS13630 (2779394) 2779394..2781097 + 1704 WP_000896263.1 protein YfjW -
LHV61_RS13635 (2781669) 2781669..2781892 + 224 Protein_2667 DUF905 domain-containing protein -
LHV61_RS13640 (2781995) 2781995..2782453 + 459 WP_000211841.1 antirestriction protein -
LHV61_RS13645 (2782462) 2782462..2782944 + 483 WP_001407480.1 RadC family protein -
LHV61_RS13650 (2782953) 2782953..2783153 + 201 WP_001395452.1 DUF987 domain-containing protein -
LHV61_RS13655 (2783191) 2783191..2783508 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LHV61_RS13660 (2783529) 2783529..2783858 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
LHV61_RS13665 (2784222) 2784222..2788802 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - luxS 2762235..2836854 74619


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T219882 WP_001094400.1 NZ_CP084894:2783529-2783858 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T219882 NZ_CP116775:4533193-4533549 [Pseudomonas sp. 273]
ATGGTGAGGCGTCCACCGAGCGTTCCCGACCGCGGTGAGATCTGGCACCTGCAGTTCGATCCCTCGACGGGCCGTGAAAT
GATCGGCGACCACTTTTGCCTGGTCCTGTCGAAACGCGAATTCAATGAAATAGGCCTGGCCCTGGTCTGCCCCATCTCGC
AAGGCACATCCAGTGTGGCGCGCAACCAGGGCTTCCTTGTCACCCTGATGGGCGAAGGCACCGACACCCAGGGCAATGTC
CACAGCCACCAGGTGCGGACGCTGGACTGGAGGGCGCGCCGAGCCATGTTCAAGGAAAAAGCCCCCGACTCCGTAACCAA
CTACGTGCTTTCGCTGGTCGGAGCGATCCTGGAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT219882 WP_000072690.1 NZ_CP084894:2783191-2783508 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT219882 NZ_CP116775:4532960-4533199 [Pseudomonas sp. 273]
ATGACCACTCTTGCCCTGCGTAAATCCGGGGGGTCCCTGATCATTTCGGTTCCCGCGGCCTTCGTCGCCCAAAACAAGCT
GCACGCCGGTAGTCAGGTTGAGTGCAACATCGTCGAGGGAGCCCTGGTGATCAAACCGATGACGCGCCCGAGCCTGGCAC
AGCTGATCGCCGAAACGCCCAAAGACTCGCTCGCTCCGGACTGGGACAACATGCAGGCCGTAGGCGCTGAGCAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References