Detailed information of TA system TA219881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2771994..2773431
• Replicon: chromosome
• Accession: NZ_CP084894
• Organism: Escherichia coli strain g83

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: LHV61_RS13580
• Protein ID: WP_000155570.1
• Coordinates: 2771994..2773067 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: LHV61_RS13585
• Protein ID: WP_000461704.1
• Coordinates: 2773060..2773431 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHV61_RS13565 (2767427)	2767427..2769616	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
LHV61_RS13570 (2769613)	2769613..2771229	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
LHV61_RS13575 (2771589)	2771589..2771852	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
LHV61_RS13580 (2771994)	2771994..2773067	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
LHV61_RS13585 (2773060)	2773060..2773431	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
LHV61_RS13590 (2773786)	2773786..2774649	+	864	WP_000787597.1	GTPase family protein	-
LHV61_RS13595 (2774741)	2774741..2775562	+	822	WP_000197391.1	DUF932 domain-containing protein	-
LHV61_RS13600 (2775779)	2775779..2776480	+	702	WP_000189409.1	WYL domain-containing protein	-
LHV61_RS13605 (2776521)	2776521..2776757	+	237	WP_001144029.1	protein YpjK	-
LHV61_RS13610 (2776757)	2776757..2777200	+	444	WP_000824222.1	lipoprotein YfjS	-
LHV61_RS13615 (2777224)	2777224..2777691	+	468	WP_000702495.1	protein YfjT	-
LHV61_RS13620 (2778078)	2778078..2778230	-	153	Protein_2664	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	luxS	2762235..2836854	74619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T219881 WP_000155570.1 NZ_CP084894:2771994-2773067 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T219881 NZ_CP116775:2795395-2795676 [Pseudomonas sp. 273]
GTGATCTACGCTCCGGCCGCGATACGCGATCTCGAATGCCTTCGGGAATACTTGCGCTCCAGGAGTCCTGATGCGGCCAG
GCGGGCTGCTCAGGCGGTCGTCCAGGCCACCCAGGCGCTGGGAAATCGCCCGCAGGTGGGACGGATAATCGAGAGCCTGC
CCGTCTCCTTCAGAGAATGGCCAATCGAGTTCGGTGATAGCGGCTATGTCGCCCGGTATCGCATCGATAACGAGACCATA
GTGATTCTCGCCATTCGGCATCAACGCGAAGCGGGCTATTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT219881 WP_000461704.1 NZ_CP084894:2773060-2773431 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT219881 NZ_CP116775:2795129-2795347 [Pseudomonas sp. 273]
ATGGCCACTTCGGTGAAGCTCGACGATGCGATGATGGCGCGCATACGCAACCTTGCGGGGATGCGCCAGCGTTCACCACA
CTGGATCATGCGTGAGGCGATTGCCCAGTATGTTGAGCGCGAGGAAAGGCGTGAGGCGCTGAATCGTGAGACGGCGGTGG
CATGGGATGAGTATCAGGGCACCGGCAAGCATGTGACGTCCGAAGCGGTCAATGAGTAG

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4