Detailed information of TA system TA219799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 872681..873186
• Replicon: chromosome
• Accession: NZ_CP084892
• Organism: Staphylococcus aureus strain CC1956/ST4614-MSSA

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: -
• Locus tag: LB315_RS04245
• Protein ID: WP_229374475.1
• Coordinates: 872893..873186 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IA75
• Locus tag: LB315_RS04240
• Protein ID: WP_001058492.1
• Coordinates: 872681..872890 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB315_RS04200 (868065)	868065..868760	+	696	WP_000520233.1	methionine ABC transporter permease	-
LB315_RS04205 (868778)	868778..869599	+	822	WP_000732334.1	MetQ/NlpA family ABC transporter substrate-binding protein	-
LB315_RS04210 (869742)	869742..870962	-	1221	WP_229374466.1	site-specific integrase	-
LB315_RS04215 (870984)	870984..871121	-	138	WP_229374467.1	hypothetical protein	-
LB315_RS04220 (871199)	871199..871897	-	699	WP_229374468.1	helix-turn-helix transcriptional regulator	-
LB315_RS04225 (872046)	872046..872258	+	213	WP_229374469.1	helix-turn-helix transcriptional regulator	-
LB315_RS04230 (872258)	872258..872530	+	273	WP_229374471.1	helix-turn-helix domain-containing protein	-
LB315_RS04235 (872542)	872542..872688	+	147	WP_229374473.1	pathogenicity island protein	-
LB315_RS04240 (872681)	872681..872890	+	210	WP_001058492.1	hypothetical protein	Antitoxin
LB315_RS04245 (872893)	872893..873186	+	294	WP_229374475.1	DUF1474 family protein	Toxin
LB315_RS04250 (873274)	873274..874143	+	870	WP_229374477.1	primase alpha helix C-terminal domain-containing protein	-
LB315_RS04255 (874157)	874157..875857	+	1701	WP_229374479.1	DUF927 domain-containing protein	-
LB315_RS04260 (876188)	876188..876568	+	381	WP_229374481.1	hypothetical protein	-
LB315_RS04265 (876565)	876565..877206	+	642	WP_229374483.1	pathogenicity island protein	-
LB315_RS04270 (877554)	877554..877895	+	342	WP_229374485.1	pathogenicity island protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vWbp	851922..884268	32346

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-97)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11421.92 Da
• Isoelectric Point: 4.9139

>T219799 WP_229374475.1 NZ_CP084892:872893-873186 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKEKINDVAIKHGWFTQETFLTNKLGTKQEHISYSVGYLEHRIQNEHTVELLQVYLKEFGELIQKF
HEIEKASLQADQSESNA

Nucleotide

• Length: 294 bp

>T219799 NZ_CP116718:c141881-141600 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8238.28 Da
• Isoelectric Point: 4.3718

>AT219799 WP_001058492.1 NZ_CP084892:872681-872890 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT219799 NZ_CP116718:c142105-141878 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: X5IA75