Detailed information of TA system TA219798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 857328..857805
• Replicon: chromosome
• Accession: NZ_CP084892
• Organism: Staphylococcus aureus strain CC1956/ST4614-MSSA

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: LB315_RS04115
• Protein ID: WP_000052076.1
• Coordinates: 857328..857516 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: LB315_RS04120
• Protein ID: WP_001059690.1
• Coordinates: 857545..857805 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB315_RS04085 (853280)	853280..853837	+	558	WP_229374463.1	hypothetical protein	-
LB315_RS04090 (854178)	854178..854864	+	687	WP_229374464.1	thermonuclease family protein	-
LB315_RS04095 (855221)	855221..855421	+	201	WP_001059082.1	cold-shock protein	-
LB315_RS04100 (855919)	855919..856137	-	219	WP_000005880.1	hypothetical protein	-
LB315_RS04105 (856199)	856199..856483	-	285	WP_001004401.1	hypothetical protein	-
LB315_RS04110 (856571)	856571..857140	-	570	WP_001106648.1	lipoprotein N-acylation protein LnsA	-
LB315_RS04115 (857328)	857328..857516	-	189	WP_000052076.1	hypothetical protein	Toxin
LB315_RS04120 (857545)	857545..857805	-	261	WP_001059690.1	hypothetical protein	Antitoxin
LB315_RS04125 (858146)	858146..858349	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
LB315_RS04130 (858346)	858346..858582	-	237	WP_000368687.1	hypothetical protein	-
LB315_RS04135 (858807)	858807..859388	+	582	WP_001140696.1	histidine phosphatase family protein	-
LB315_RS04140 (859461)	859461..860078	-	618	WP_000255553.1	LysE/ArgO family amino acid transporter	-
LB315_RS04145 (860235)	860235..860729	-	495	WP_000569881.1	GNAT family N-acetyltransferase	-
LB315_RS04150 (861116)	861116..861538	-	423	WP_103204578.1	organic hydroperoxide resistance protein	-
LB315_RS04155 (861686)	861686..862402	+	717	WP_000150028.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vWbp	851922..884268	32346

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-69)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T219798 WP_000052076.1 NZ_CP084892:c857516-857328 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T219798 NZ_CP116717:c6069916-6069638 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT219798 WP_001059690.1 NZ_CP084892:c857805-857545 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT219798 NZ_CP116717:c6069626-6069321 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56